首页|Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus
Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus
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国家科技期刊平台
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The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology.The roughskin sculpin(Trachidermus fasciatus Heckel),displaying sexual dimorphism,provides an ideal model for studying the mechanisms.However,both genetic and genomic information concerning sex determination and differentiation,such as gonadal transcriptome data in roughskin sculpin,are lacking.Here,we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes.We identified 8 531 differentially expressed genes(DEGs),among them 4 065 were upregulated in the ovary and 4 466 upregulated in the testis.Several sex-related gene ontology(GO)terms were enriched in ovary-biased genes,including"binding of sperm to zona pellucida","egg coat formation","positive regulation of acrosome reaction","cell division",and"cell cycle",while the GO terms such as"spermatogenesis","sperm axoneme assembly","cilium assembly","cilium movement",and"cilium movement involved in cell motility"were enriched in testis-biased genes.Moreover,six KEGG pathways were significantly enriched in the ovary,whereas only one was enriched in the testis.Of these DEGs,40 sex-related genes were identified,which including 26 testis-biased genes(such as Dmrtb1,Gsdf Sox9b,Wnt4b,Tcp11l2,and Efhb),and 14 ovary-biased genes(such as Cyp19a1a,Foxh1,Foxr1,Gdf3,Hsd17b12,and Igf2bp3).This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin,expand the genomic database,support future studies on sex-related gene functions,and facilitate molecular biology research into roughskin sculpin.
Dongxiu XUE、Sudong XIA、Tengfei XING、Yuqian GAO、Jinxian LIU
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CAS Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China
Laboratory for Marine Ecology and Environmental Science,Qingdao Marine Science and Technology Center,Qingdao 266200,China
Tianjin Key Lab of Aqua-Ecology and Aquaculture,Department of Fishery Science,Tianjin 300380,China
National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaKey Scientific Research Project in Universities and Colleges in Tianjin
国家科技期刊平台
NSTL
万方数据
