基于Bld-1短肽构建靶向膀胱癌工程化外泌体的研究
Construction of Engineered Exosomes Targeting Bladder Cancer Based on Bld-1 Short Peptide
刘戴胤 1郑汉豪 1陈健成 1林彦 1安明杰 1陈长昊1
作者信息
- 1. 中山大学孙逸仙纪念医院泌尿外科,广州 510120
- 折叠
摘要
探讨基于Bld-1短肽构建靶向膀胱癌的工程化外泌体.利用Bld-1短肽,通过构建过表达质粒,使其与LAMP2B蛋白融合表达,从而构建LAMP2B/Bld-1的工程化外泌体,并进一步通过外泌体摄取实验探讨其在膀胱癌中应用的可行性.实验结果显示,LAMP2B/Bld-1工程化外泌体能够高效靶向膀胱癌细胞系,且可以作为药物载体,将药物直接送达膀胱癌细胞.通过Bld-1短肽构建靶向膀胱癌的工程化外泌体具有可行性,为膀胱癌的治疗提供了新的研究方向和治疗策略.
Abstract
This paper focused on the construction of engineered exosomes targeting bladder cancer based on Bld-1 short peptide.Using the short peptide Bld-1,the engineered exosomes of LAMP2B/Bld-1 were constructed by transfecting with LAMP2B/Bld-1 fusing expression plasmids.The feasibility of LAMP2B/Bld-1 engineered exosomes application in bladder cancer was further evaluated through exosome uptake experiment.The results showed that the LAMP2B/Bld-1 engineered exosomes could specifically target bladder cancer cell lines and serve as drug carriers to deliver drugs directly to bladder cancer cells.It is feasible to construct engineered exosomes targeting bladder cancer cells based on Bld-1 short peptide,which provides a new research direction and treatment strategy for patients with bladder cancer.
关键词
Bld-1短肽/膀胱癌/工程化外泌体/LAMP2B蛋白Key words
Bld-1 short peptide/bladder cancer/engineered exosomes/LAMP2B protein引用本文复制引用
基金项目
国家重点研发计划青年科学家项目(2022YFA1305500)
出版年
2024
NETL
NSTL
万方数据