Research Progresses on Three Dimensional Super-Resolution Imaging via Cylindrical Lens
Three dimensional super-resolution imaging technique has been widely used in intracellular imaging of sub-micrometer structures,but its developments and applications require a precise calibration.The cylindrical lens is one of the key optics in three dimensional super-resolution imaging.Study of how the distance between cylindrical lens,camera imaging plane,and the microscope tube lens remains to be a significant task on three dimensional super-resolution imaging.Based on these,this paper focused on the investigations of several key elements that determine the calibration of three dimensional super-resolution imaging.Through careful calibration on how camera-lens distance impacted the imaging system,this paper achieved super-resolution imaging of intracellular membrane structures on three dimensions,and spatially mapped the diffusion rates along x-y direction and z axis at the nanoscale.Taken together,these results provide a detailed protocol on the calibration of three dimensional super-resolution imaging system,and pave the way for its wider application in intracellular imaging.
three dimensional super-resolution imagingcylindrical lensdiffusion rate
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