首页|Geometric phase helical PSF for simultaneous orientation and 3D localization microscopy
Geometric phase helical PSF for simultaneous orientation and 3D localization microscopy
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The 3D location and dipole orientation of light emitters provide essential information in many biological,chemical,and physical systems.Simultaneous acquisition of both information types typically requires pupil engineering for 3D localization and dual-channel polarization splitting for orientation deduction.Here we report a geometric phase helical point spread function for simultaneously estimating the 3D position and dipole orientation of point emitters.It has a compact and simpler optical configuration compared to polarization-splitting techniques and yields achromatic phase modulation in contrast to pupil engineering based on dynamic phase,showing great potential for single-molecule orientation and localization microscopy.
PSF engineeringgeometric phasesingle-molecule orientation and localization microscopy
周勇壮、张洪硕、沈咏、Andrew R.Harvey、邹宏新
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Institute for Quantum Science and Technology,College of Science,National University of Defense Technology,Changsha 410073,China
Hunan Key Laboratory of Mechanism and Technology of Quantum Information,Changsha 410073,China
School of Physics and Astronomy,University of Glasgow,Glasgow G12 800,UK
国家自然科学基金国家自然科学基金国家自然科学基金Science and Technology Innovation Program of Hunan Province
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