Stimulated emission-depletion-based point-scanning structured illumination microscopy

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外文摘要：Wide-field linear structured illumination microscopy[LSIM]extends resolution beyond the diffraction limit by moving unre-solvable high-frequency information into the passband of the microscopy in the form of moiré fringes.However,due to the diffraction limit,the spatial frequency of the structured illumination pattern cannot be larger than the microscopy cutoff frequency,which results in a twofold resolution improvement over wide-field microscopes.This Letter presents a novel approach in point-scanning LSIM,aimed at achieving higher-resolution improvement by combining stimulated emission depletion[STED]with point-scanning structured illumination microscopy[psSIM][STED-psSIM].The according structured illumination pattern whose frequency exceeds the microscopy cutoff frequency is produced by scanning the focus of the sinusoidally modulated excitation beam of STED microscopy.The experimental results showed a 1.58-fold resolution improvement over conventional STED microscopy with the same depletion laser power.

外文关键词：

stimulated emission depletionstructured illumination microscopysuperresolution microscopy

作者：

汪磊、王美婷、王璐玮、郑晓敏、陈嘉杰、吴文帅、严伟、于斌、屈军乐、高志、邵永红

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作者单位：

Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging,National-Regional Key Technology Engineering Laboratory for Medical Ultrasound,School of Biomedical Engineering,Shenzhen University Medical School,Shenzhen 518060,China

Key Laboratory of Opto-electronic Information Science and Technology of Jiangxi Province,Nanchang Hangkong University,Nanchang 330063,China

College of Physics and Optoelectronics Engineering,Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,Shenzhen University,Shenzhen 518060,China

Department of Bioengineering and COMSET,Clemson University,Clemson SC 29634,US

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基金：

国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家重点研发计划Guangdong Natural Science Foundation and Province ProjectGuangdong Natural Science Foundation and Province ProjectFoundation from Department of Science and Technology of Guangdong ProvinceFoundation from Department of Education of Guangdong ProvinceShenzhen Science and Technology Research and Development and Innovation FoundationShenzhen Key Laboratory of Photonics and BiophotonicsMedical-Engineering Interdisciplinary Research Foundation of Shenzhen University

项目编号：

622751686227516461775148619051452022YFA12063002021A15150119162023A15150122502021QN02Y1242023ZDZX2052JCYJ20200109105608771ZDSYS20210623092006020

出版年：

2024

DOI：

10.3788/COL202422.031701

中国光学快报(英文版)

中国光学学会中国科学院上海光学精密机械研究所

中国光学快报(英文版)

CSTPCD

影响因子：1.305

ISSN：1671-7694

年,卷(期)：2024.22(3)

参考文献量29