首页|Iterative multi-photon adaptive compensation technique for deep tissue two-photon fluorescence lifetime imaging

Iterative multi-photon adaptive compensation technique for deep tissue two-photon fluorescence lifetime imaging

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Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively.However,the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological tissues.This paper introduces an iterative multi-photon adaptive compensation technique[IMPACT]into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep tissues.It shows that 400 correction modes can be achieved within 5 min,which was mainly limited by the frame rate of a spatial light modulator.This system was used for high-resolution imaging of mice brain tissue and live zebrafish,further verifying its superior performance in imaging quality and photon accumulation speed.

adaptive opticsiterative optimizationtwo-photon fluorescence lifetime imaging microscopywavefront correction

王柯欣、余文慧、屈军乐、廖常锐、王义平、何俊、刘丽炜

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State Key Laboratory of Radio Frequency Heterogeneous Integration[Shenzhen University],Shenzhen 518060,China

Key Laboratory of 0ptoelectronic Devices and Systems of Guangdong Province & Ministry of Education,College of Physics and 0ptoelectronic Engineering,Shenzhen University,Shenzhen 518060,China

National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaShenzhen Key ProjectsShenzhen Talent Innovation ProjectShenzhen Science and Technology Planning Project

2021YFF0502900621751636222550561935012618350096212781962205220JCYJ20200109105404067RCJC20210706091949022ZDSYS20210623092006020

2024

10.3788/COL202422.041702

中国光学快报(英文版)
中国光学学会 中国科学院上海光学精密机械研究所

中国光学快报(英文版)

CSTPCD
影响因子：1.305
ISSN：1671-7694
年,卷(期)：2024.22(4)