Study on Effect of Implantable Photobiomodulation on Axonal Regeneration and Motor Function Recovery of Mice Suffered Spinal Cord Injury
Objective To investigate the effect of implantable biomedical fiber-optic-mediated photobiomodulation(PMB)on the axonal regeneration and motor function recovery in spinal cord injured(SCI)mice.Methods The research subjects included 45 C57BU6J mice,those were randomly divided into 3 groups(15 mice in each group):a sham operation(Sham)group,spinal cord injury(SCI)group and spinal cord injury+implantable photobiomodulation therapy(SCI+PBM)group.The mice in the Sham group were given only laminectomy of the T9 vertebral body.The mice in the SCI group and the SCI+PBM group all experienced the construction of a spinal cord clamp injury model upon the laminectomy of the T9 vertebral body,after which the implantable biomedical optical fiber was fixed on the spinous processes and paravertebral soft tissues of T8 and T10 vertebrae in the mice with spinal cord injury.No PBM intervention was given to the mice in the SCI group.For the mice in the SCI+PBM group,low-energy laser irradiation treatment with a wavelength of 808 nm,a power density of 50 mW/cm2 and a duration of 30 minutes was given immediately after the spinal cord injury,which was conducted at the same time per day for 28 days in total.On the 14th day after the intervention on the mice of the three groups,4 mice were taken from each group,1 cm spinal cord tissue was taken from their T9 segment of the spinal cord as the center to make frozen sections,and immunofluorescence staining was used to observe the change to the number of the Oligo2+oligodendrocytes and Map2+neurons in the injured areas with a view to evaluating the axonal regeneration of the mice in the three groups.On the 1st,3rd,7th,14th,21th and 28th day after the intervention on the mice of the three groups,basso mouse scale(BMS)scores were used to evaluate the recovery of hind limb motor functions of 6 mice picked from each group respectively.On the 28th day after the intervention,motor evoked potential detection was conducted on 4 mice of each group to evaluate the recovery of the mice's spinal cord injury.Results The number of neuronal axons and oligodendrocytes involved in myelination formation in the injured area was much smaller in the SCI group than in the Sham group(P＜0.05),but the number was much greater in the SCI+PBM group than in the SCI group(P＜0.05).The evaluation of mouse motor function showed that the BMS score and motor evoked potential amplitude of the mice in the SCI+PBM group were higher than those in the SCI group(P＜0.05).Conclusions The implantable biomedical fiber-optic-mediated photobiomodulation(PMB)will promote the survival of oligodendrocytes,axonal regeneration and motor function recovery in SCI mice.
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