630 nm LED无创光疗干预痛风性关节炎的基础研究
Fundamental Study on Noninvasive Phototherapy Intervention of Gouty Arthritis with 630 nm LED
张敏 1刘成波 2谭一舟 3王颖 3邱海霞 3曾晶 3顾瑛1
作者信息
- 1. 解放军医学院(北京市,100853);中国人民解放军总医院第一医学中心激光医学科
- 2. 中国科学院深圳先进技术研究院
- 3. 中国人民解放军总医院第一医学中心激光医学科
- 折叠
摘要
目的 初步探索波长630 nm窄带LED无创光疗干预大鼠痛风性关节炎的效应.方法 实验对象SD大鼠24只,随机分为对照组、痛风组和光疗组三组,每组大鼠各8只.三组先进行痛风性关节炎模型诱导,其中对照组大鼠右踝注射生理盐水0.25 ml;痛风组和光疗组大鼠右踝注射50mg/ml尿酸钠悬浊液0.25 ml,各组注射后4 h,进行关节炎症评分、肿胀度测量、步态评分和足回缩阈值检测以判定建模情况.对照组、痛风组诱导建模后均不进行LED无创光疗干预;光疗组痛风性关节炎建模成功后,采用波长630 nm的LED无创光疗干预,照射功率密度10 mW/cm2,每次照射时间1h,每日1次,连续照射15d.LED照射后,每日对三组大鼠进行炎症外观(炎症评分和肿胀度)、行为学(步态评分和足回缩阈值)观察,其中足回缩阈值采用von Frey细丝进行机械触痛评价.同时检测三组大鼠在建模前和LED照射后第1天、第3天、第7天、第11天、第15天血清中炎症因子IL-1β、TNF-α水平.结果 (1)模型评估:注射后4 h,痛风组和光疗组关节红肿,炎症评分、肿胀度显著高于对照组(P＜0.05);与对照组比较,痛风组和光疗组大鼠均出现行为障碍,步态评分增加(P＜0.05)、足回缩阈值降低(P＜0.05),提示造模成功.(2)炎症外观改变:LED照射干预前,痛风组与光疗组的炎症评分和肿胀度比较差异无统计学意义(P＞0.05).建模后第1天,痛风组和光疗组炎症评分分别为(5.38±0.7)分和(4.56±0.5)分,肿胀度分别为(27.1±1.1)％和(21.5±1.2)％,两组比较差异均具有统计学意义(P＜0.05).痛风组建模后第10天炎症评分、第12天肿胀度与建模前比较差异无统计学意义(P＞0.05),且与对照组比较差异无统计学意义(P＞0.05),第11天炎症评分、第13天肿胀度恢复至正常水平;光疗组建模后第8天炎症评分和肿胀度与建模前比较差异无统计学意义(P＞0.05),且与对照组比较差异无统计学意义(P＞0.05),第9天两项指标均恢复正常.(3)行为学改变:在LED照射干预前,痛风组与光疗组步态评分和足回缩阈值比较差异无统计学意义(P＞0.05).光疗组LED照射后第1天,痛风组和光疗组步态评分分别为(5.63±0.7)分和(4.15±0.8)分,足回缩阈值分别为(0.94±0.6)g和(1.98±1.0)g,两组比较差异均具有统计学意义(P＜0.05).痛风组建模后第8天步态评分、第9天足回缩阈值建模前比较差异无统计学意义(P＞0.05),与对照组比较差异无统计学意义(P＞0.05);第10天步态评分、足回缩阈值恢复正常;而光疗组LED照射后第6天步态评分和足回缩阈值与建模前比较差异无统计学意义(P＞0.05),与对照组比较差异无统计学意义(P＞0.05),第8天步态评分、第7天足回缩阈值恢复正常.(4)炎症因子水平改变:建模后第1天痛风组和光疗组IL-1β、TNF-α水平均明显高于对照组(P＜0.05);光疗组LED照射后第1天,IL-1β、TNF-α水平明显低于痛风组(P＜0.05);LED照射后第7天,光疗组IL-1β、TNF-α水平与对照组比较,差异均具有统计学意义(P＜0.05).结论 波长630 nm窄带LED无创光疗可有效缓解痛风性关节炎大鼠的肿胀和疼痛症状,是一种无创、无毒副作用、简便易行、安全有效的干预方法.
Abstract
Objective To investigate the impact of the noninvasive phototherapy with 630 nm narrowband LED on gouty arthritis in rats. Methods Totally 24 Sprague-Dawley rats were randomly divided into three groups:control,gout and light group,8 rats in each group.The rats in the control group were given standard feeding and a normal saline injection in the right ankle.Given no light therapy,the rates in the gouty group were induced with gouty arthritis by injecting sodium urate suspension into their right ankles.Upon gout induction,the rats in the light group were given the noninvasive phototherapy with 630 nm LED(power density:10 mW/cm2)for an hour per day,which lasted for 15 days in total.The daily assessments for the rats included joint inflammation scoring,swelling measurement,von frey filaments testing for foot retraction threshold and evaluation of mechanical tenderness.Blood samples were collected for all the rats from the tail vein at various time points(1d,3d,7 d,11d and 15 d)for ELISA analysis of serum IL-1β and TNF-α inflammatory factors during the acute inflammatory phase.Results(1)Model evaluation:4h after the injection,the joint redness and swelling,inflammation score and swelling degree were greater in the gout group and phototherapy group than in the control group(P＜0.05),and behavioral disorder level,gait score and von frey threshold higher in the gout group and phototherapy group(P＜0.05),indicating a successful modeling.There was no statistical difference in each index(P＞0.05).(2)Inflammatory appearance changes:On the first day after the modeling,the inflammation scores and swelling degrees in the gout group and phototherapy group were(5.38±0.7)and(4.56±0.5),and(27.1±1.1)％and(21.5±1.2)％respectively,and the differences between the two groups were significant(P＜0.05).In the gout group,the inflammation score on the 10th day after the modeling and the swelling degree on the 12th day were not remarkably different from those before the modeling(P＞0.05).For the phototherapy group,the inflammation score and swelling degree on the 8th day after the modeling were also not much different from those before the modeling(P＞0.05).(3)Behavioral changes:On the first day after the modeling,the gait scores and threshold of foot retraction of the gout group and phototherapy group were(5.63±0.7)and(4.15±0.8),and(0.94±0.6)g and(1.98±1.0)g respectively,and the differences were statistically significant(P＜0.05).In the gout group,the gait score and swelling recovered to the level before the modeling on the 6th and 8th day after the modeling respectively,and the differences between the gout group and control group were not significant(P＞0.05).For the phototherapy group,the gait score and swelling recovered to the level before the modeling on the 6th and 9th day after the modeling respectively,and the differences between the phototherapy group and control group were not significant(P＞0.05).(4)Results of inflammatory factors:On the 1st day after the modeling,the IL-1β and TNF-α levels were much higher in the gout group and phototherapy group than in the control group on the 1st day after the modeling(P＜0.05),and the IL-1β and TNF-α levels much lower in the phototherapy group than in the gout group(P＜0.05).On the 7th day after the modeling,the IL-1β and TNF-α levels of the photography group recovered to the normal levels.Conclusions Noninvasive phototherapy is effective in reducing swelling and alleviating pain in rats with gouty arthritis.Being non-invasive,non-toxic,straightforward and safe,it is a promising and efficacious approach for managing gouty arthritis.
关键词
无创光疗/痛风性关节炎/发光二极管/疼痛/肿胀Key words
Noninvasive phototherapy/Gouty arthritis/Light-emitting diode/Pain/Swell引用本文复制引用
基金项目
中国医科院医学与健康科技创新工程项目(2019-I2M-5-061)
出版年
2024
NETL
NSTL
万方数据