Study Based on NLRP3 Inflammasome:Effect and Mechanism of Nd:YAP Laser in Treating Periodontitis
Objective To investigate the effect and possible mechanism of Nd:YAP laser in the treatment of periodontitis based on NLRP3 inflammasome.Methods Totally 140 patients with periodontitis treated from Sep.2022 to 2023 Sep.were selected and divided randomly into a control group and Nd:YAP laser group,70 patients in each group.The patients in both groups were firstly treated with ultrasonic supringival cleaning and subgingival curetting.Then the patients in the Nd:YAP laser group were given 3％hydrogen peroxide solution for rinsing periodontal bags and Nd:YAP laser intraperiodontal bag irradiation while the patients in control group being given only 3％hydrogen peroxide solution for rinsing periodontal bags.The periodontal health,level of inflammatory factors in gingival crevicular fluid and short-term curative effect were compared between the two groups,and the influence of Nd:YAP laser treatment on NLRP3 inflammasome was analyzed.Results For both groups,the PD and GI were lower 6 and 12 weeks after the treatment than before the treatment(P＜0.05).The PD and GI in the Nd:YAP laser group were lower than those in the control group(P＜0.05).The levels of IL-6,TNF-α and CRP in gingival crevicular fluid were lower in the Nd:YAP laser group than in the control group(P＜0.05).Twelve weeks after the treatment,Nd:YAP laser group had higher efficacy class and total effective rate than the control group(P＜0.05).The expression levels of NLRP3 mRNA,ASC mRNA and caspase-1 mRNA in gingival crevicular fluid in the Nd:YAP laser group were lower than those in the control group 6 and 12 weeks after the treatment(P＜0.05).Conclusions Addition of Nd:YAP laser to the overall treatment of periodontitis patients will further improve the periodontal health status of patients,reduce inflammation and enhance short-term curative effect.The realization of this effect may be related to the effect of Nd:YAP laser on inhibiting the activation of NLRP3 inflammasome.
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