Spermatogenesis failure and infertility of mice caused by the deficiency of peroxisome biogenesis factor
Objective:The peroxisome biogenesis factor 5(PEX5)was a key factor of the peroxisome biogenesis,which was high expression in the testis of the male reproductive system.The aim of this study was to investigate the influence of PEX5 of male mice on their spermatogenesis and fertility.Methods:The model mice with the testis-specific Pex5 knockout(Pex5 conditional knockout,Pex5 cKO)were constructed by CRISPR/Cas9 and LoxP/Cre technology.The reproductive organs and spermatogenesis of the male mice were assessed by hematoxylin-eosin staining(HE),immu-noprotein blotting(Western blot)and immunofluorescence(IF)staining.Results:The litter size of the males with Pex5 cKO was 0,and which was significantly less than that(6.32±0.26)of the wild type(WT)male mice(t=21.46,P＜0.0001).The HE staining results showed no spermatogenesis in the epididymis of the mice with Pex5 cKO.Conclusion:Pex5 plays an important role in the mice spermatogenesis.
万方数据
维普
