Value of four-dimensional ultrasound combined with the serum circular RNA homeodomain-interacting protein kinases 3 and circular RNA 003416 of pregnant women for diagnosing the congenital heart disease of their fetuses
Objective:To explore the value of four-dimensional ultrasound combined with the levels of serum circular RNA homeodomain-interacting protein kinases 3(circHIPK3)and circular RNA 003416(circ0003416)of pregnant women for diagnosing the congenital heart disease(CHD)of their fetuses.Methods:The clinical data of 324 pregnant women with the suspected of fetal CHD from November 2021 to November 2023 were collected.These women were di-vided into study group(43 fetuses with CHD)and control group(281 fetuses without CHD)based on the postpartum follow-up results or the pathological results after induced abortion as the gold standard.The general data of the women in the two groups were collected.Real-time fluorescence quantitative PCR(qRT PCR)method was applied to detect the levels of serum circHIPK3 and circ0003416 of the women in the two groups.The results of four-dimensional ultra-sound examination of the women in the two groups were recorded.Kappa was used to analyze the consistency between the four-dimensional ultrasound combined with the levels of circHIPK3 and circ0003416 of the women and the gold standard for diagnosing the fetal CHD.Receiver operating characteristic(ROC)curve was used to analyze the diagnos-tic value of the four-dimensional ultrasound combined with the levels of circHIPK3 and circ0003416 of the women for their fetal CHD.Results:The serum circHIPK3 level(1.41±0.18)of the women in the study group was significantly higher than that(1.19±0.13)of the women in the control group,and the serum circ0003416 level(0.82±0.11)of the women in the study group was significantly lower than that(1.03±0.14)of the women in the control group(all P＜0.05).The consistency between the four-dimensional ultrasound of the women for diagnosing their fetal CHD and the final diagnostic result by the gold standard was better(Kappa=0.809,P＜0.001).The area under the curve(AUC)of the four-dimensional ultrasound,the serum circHIPK3 level or the serum circ0003416 level of the women for diagnosing their fetal CHD was high.The AUC and the sensitivity of the combined four-dimensional ultrasound and the levels of serum circHIPK3 and circ0003416 of the women for diagnosing their fetal CHD were 0.976 and 97.7％,and which were the highest(P＜0.001).Conclusion:The maternal serum circHIPK3 level of the women with fetal CHD increases,while their serum circ0003416 level decreases.The combination of the four-dimensional ultrasound and the levels of serum circHIPK3 and circ0003416 levels of the pregnant women for their fetal CHD has higher diagnostic val-ue,which can provide the references for the clinical screening of CHD.
Congenital heart diseaseFour-dimensional ultrasoundCircular RNA homeodomain interacting protein ki-nase 3Circular RNA0003416Diagnostic value
万方数据
维普
