Expressions and clinical significances of intercellular adhesion molecule-1 and hypoxia-inducible factor-1a in the endome-trial tissue of infertility patients with endometriosis
Objective:To analyze the expressions and the clinical significances of intercellular adhesion molecule-1(ICAM-1)and hypoxia-inducible factor-1α(HIF-1α)in the endometrial tissue of infertility patients with endometriosis(EMs).Methods:A total of 85 infertile patients with EMs who had undergone laparoscopic surgery from April 2021 to April 2023 were selected in observation group,and among them,there were 46 cases with stage Ⅰ-Ⅱ of Ems in group A and 39 cases with stage Ⅲ-Ⅳ of Ems in group B according to the American fertility society staging criteria(r-AFS).In addition,70 patients with infertility because of simple tubal factor who had undergone hysteroscopy during the same time were selected in control group.The expressions of ICAM-1 mRNA and HIF-1α mRNA in the endometrial tissues of the patients in the two groups were detected by reverse transcription polymerase chain reaction(RT-PCR)method.The expressions of ICAM-1 protein and HIF-1α protein in the endometrial tissues of the patients in the two groups were detected by Western blot method.The serum ICAM-1 and HIF-1α levels of the patients in the two groups were detected by ELISA.Receiver operating characteristic(ROC)curve was plotted to analyze the predictive values of the ICAM-1 and HIF-1α levels of the patients with EMs for their infertility.The correlation between the ICAM-1 level of the infertility patients with EMs and their HIF-1αlevel.Results:The expressions of ICAM-1 and HIF-1αmRNA in the endometrial tissue(2.71±0.22 and 0.87±0.23)of the patients in the observation group were significantly higher than those(0.37±0.12 and 0.47±0.15)of the patients in the control group.The expressions of ICAM-1 and HIF-1α protein(2.37±0.07 and 0.81±0.20)of the patients in the observation group were significantly higher than those(0.10±0.03 and 0.35±0.10)of the patients in the control group.The levels of serum sICAM-1 and HIF-1α(278.42±32.05 ng/ml and 90.95±10.77 ng/ml)of the patients in the observation group were significantly higher than those(194.60±19.55 ng/ml and 11.03±3.34 ng/ml)of the patients in the control group.The serum levels of sICAM-1 and HIF-1α of the patients in group B were significantly higher than those of the patients in group A(all P＜0.05).ROC curve analysis showed that the area under the curve,the sensitivity and the specificity of the levels of serum sICAM-1 and HIF-1α of the patients with EMs for predicting their infertility were 0.865,92.0％and 65.0％,respectively,and which were sig-nificantly higher than those of the serum sICAM-1 level or the serum HIF-1α level alone(all P＜0.05).Pearson corre-lation analysis showed that there was no correlation between the ICAM-1 mRNA level of the patients and their HIF-1αmRNA level,HIF-1α protein level or serum HIF-1αlevel(all P＞0.05).Conclusion:The abnormal expression of ICAM-1 and HIF-1α of the patients with EMs may play an important role in the occurrence and development of their infertility.The levels of serum ICAM-1 and HIF-1α of the infertility patients increase with the severity of their EMs,and which have certain value for predicting the infertility of the patients because of EMs.
Infertility because of endometriosisIntercellular Adhesion Molecule-1Hypoxia-Inducible Factor-1αEn-dometrial tissuePredictionCorrelation
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维普
