Objective To analyze the current infection status of Helicobacter pylori(Hp)and its influencing fac-tors among persons who took physical examination,and to provide reference for clinical prevention and treatment of Hp infec-tion.Methods A simple random sampling method was used to select part of persons who took physical examination in Tsing-hua University Hospital as the research objects.Urea[13 C]breath test was used to detect Hp infection.Binary logistic re-gression model was used to analyze the main factors associated with Hp infection.Results There were 415 cases of Hp infec-tion in the physical examination population,and the Hp infection rate was 29.94%.Binary logistic regression analysis results showed the risk of Hp infection in persons with abnormal Hcy was higher,and the OR value was 1.941(95%CI:1.154-3.262).Compared to persons without alcohol drinking as reference,the risk of Hp infection was higher in persons who drank alcohol,OR was 2.404(95%CI:1.213-4.764).Compared to persons who did not eat outside often as reference,the risk of Hp infection was higher in persons who often ate outside,OR was 1.842(95%CI:1.188-2.858).Compared to persons without Hp family history as reference,the risk of Hp infection was higher persons with family history of Hp,OR was 1.650(95%CI:1.077-2.530).Compared to persons who had better cognition of Hp infection as reference,the risk of Hp infection was higher in persons with poor cognition of Hp infection,OR was 2.418(95%CI:1.155-5.063).Conclusion There are many factors affecting Hp infection in healthy people,and there are also some deficiencies in the cognition related to Hp infection.In the future,relevant departments should take targeted prevention and intervention meas-ures.