Effective Inactivation of P.aeruginosa and ARGs Removal in UV/PAA Process:Performance and Mechanisms
Peracetic acid(PAA)can effectively inactivate bacteria and control disinfection byproducts(DBPs).However,it may promote bacterial reactivation after treatment.This study investigated the performance and mechanism of the UV/PAA process for P.aeruginosa inactivation to enhance disinfection.Experimental and characterization methods such as inactivation,dark repair,membrane permeability analysis and electron microscopy scanning showed that the inactivation performance of the UV/PAA process was superior to that of UV or PAA alone due to the generation of free radicals and intracellular reactive oxygen species(ROS)in the UV/PAA system.The membrane permeability of P.aeruginosa increased over time when exposed to the UV/PAA system.Additionally,severe disruption of the cell surface structure was observed,resulting in the leakage of intracellular material such as DNA and proteins.The UV/PAA treatment was highly effective in inhibiting the bacterial dark repair phenomenon and further inactivating the viable but non-culturable(VBNC)cells,demonstrating a far superior oxidation ability of UV or PAA alone.Additionally,the UV/PAA process could efficiently eliminate antibiotic resistance genes(ARGs).
ultravioletperacetic acidinactivationP.aeruginosaviable but non-culturable(VBNC)antibiotic resistance genes
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