Application of Loop-Mediated Isothermal Amplification in Detection of Foodborne Pathogens
A novel method called loop-mediated isothermal amplification(LAMP)has been developed in recent years.The method could be completed under isothermal conditions(60-65℃)in 1 hour by employing 4-6 primers recognizing 6-8 distinct sequences of the target gene.LAMP has been widely used in nucleic acid detection with its high sensitivity,specificity and rapidity.In this article,the technical principles and characteristics,analytical methods of amplification products and its application in the detection of several foodborne pathogens are reviewed.Future perspectives toward wider applications of LAMP assays in foodborne pathogens testing are discussed.
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