Application,Challenges and Countermeasures of Nucleic Acid Amplification Technology in Animal Quarantine
Nucleic acid amplification technology is a widely used technique in the field of biotechnology,which amplifies DNA or RNA fragments to million-fold or even higher folds through specific methods,allowing these fragments to be tested and identified.Nucleic acid amplification technology includes various methods,such as quantitative real-time PCR(qPCR),digital PCR(dPCR),recombinase polymerase amplification(RPA),etc.In animal quarantine,by amplifying nucleic acids of specific pathogens in animals,the pathogen can be quickly and accurately detected,and corresponding prevention and control measures can be taken.Through nucleic acid amplification of pathogenic microorganisms in animal-derived food,harmful substances such as bacteria and viruses in food can be detected to ensure food safety.With the development of technology,nucleic acid amplification technology will become more automated,high-throughput and fast,and the sensitivity and specificity will be further improved.With the development of microfluidic technology and nanotechnology,combined with artificial intelligence,big data and other technologies,nucleic acid amplification technology will become more portable,to achieve intelligent and information-based detection.Multidisciplinary intersection will become an important trend in the development of nucleic acid amplification technology,which will promote the innovation and application of this technology.
NETL
NSTL
万方数据
