DNA Barcode Identification System Establishment and Optimization of Quarantine Weed Cenchrus Spinifex Cav
To overcome the limitations of morphological identification of the quarantine weed Cenchrus spinifex at different de-velopmental stages and with different morphological characteristics,the amplification and sequencing efficiencies of the sequences were compared by designing primers for the DNA barcodes matK,ITS,rbcL,psbA,and trnL sequences of Cenchrus spinifex and optimizing their PCR reaction systems.The results showed that the success rates of amplification and sequencing of Cenchrus spinifex were high with primers of matK,ITS,rbcL,psbA and trnL,and the optimal annealing temperatures for PCR reaction were matK 56℃,ITS 60℃,rbcL 60℃,psbA 60℃,trnL 60℃.The objective bands obtained under the above reaction system and conditions are clear and bright,and matK,ITS,rbcL,psbA and trnL can be used as a combination DNA barcode reference sequence to identify Cenchrus Spinifex Cav.
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