Optimization of Oil Degradation Conditions by Sphingosinomonas and Bacillus subtilis
Petroleum hydrocarbon pollution has been widely concerned because of its long duration and difficulty in pollution removal.How to optimize the degradation conditions of petroleum components and effectively remove petroleum hydrocarbon pollution has been paid increasing attention at home and abroad.The outer membrane of Sphingosinomonas does not contain Lipopolysaccharides,but it does contain a Glycosphingolipid that forms a hydrophobic surface,which is more conducive to the uptake of extracellular substances.In this experiment,diesel fuel that is easily degraded by microorganisms was selected as the carbon source,and the temperature,oscillator speed,pH,concentration of petroleum pollutants,amount of bacteria,surfactant and other conditions were optimized for the degradation of diesel by Sphingomonas polyaromaticivorans.The experimental results showed that the degradation effect of Sphingomonas polyaromaticivorans was better when the concentration of diesel fuel was 0.1 g/L,35℃,pH = 7.2,and the rotation speed was 220 r/min-1.In view of the characteristics of Bacillus subtilis that can secrete surfactants,the feasibility of co-degradation of Sphingosinomonas and Bacillus subtilis was analyzed,including using biochar as a carrier to prevent bacterial interference,in order to improve the degradation efficiency in subsequent experiments.
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