Establishment of a Visual Nucleic Acid Strip Test for Viral Haemorrhagic Septicaemia Virus
A rapid and visual nucleic acid test strip assay for Viral Haemorrhagic Septicaemia Virus(VHSV)was successfully established in this study.This was achieved by designing a pair of conserved primers at the 5'end of the conserved region of VHSV and modifying biotin and fluorescein isothiocyanate(FITC)at the 5'end of the upstream and downstream primers,respectively.The method has high sensitivity and is able to detect VHSV at 33 copies/μL,which is a 10-fold increase in sensitivity over the gel imaging detection method.The experimental results confirmed that the method had simple operating procedures,rapid response,sensitivity,and good specificity,which could meet the needs of the field for rapid detection of VHSV,and provided a new methodological basis for epidemiological investigation and prevention and control.
Viral Haemorrhagic Septicaemia Virus(VHSV)nucleic acid test stripsvisual detection
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