Development of Colloidal Gold Antibody Detection Kit for Dengue Virus
To establish a colloidal gold antibody test strip for detecting dengue virus(DENV),we conducted antigen sequence selection and homology analysis.Subsequently,we specifically selected the specific antigen of dengue virus non structural protein 1(NS1)with high specificity for antibody detection.We then conducted tests to evaluate its specificity,sensitivity,repeatability,stability,anti-interference and anti-destruction properties.The results showed that the specificity and interference test of the prepared colloidal gold anti physical examination test agent strip met the requirements of yield and quality,and there was no cross reaction with St.Louis encephalitis virus(SLEV),West Nile virus(WNV),Japanese encephalitis virus(JEV),or yellow fever virus(YFV).Furthermore,the kit was not affected by serum matrix such as dry triglyceride and bilirubin.Even after being stored at 37℃for 1 month,it still met the product quality standards,demonstrating good sensitivity.The study successfully developed a colloidal gold antibody test strip for the rapid detection of dengue virus,characterized by specificity,sensitivity,repeatability,anti-interference and anti-destruction properties,providing a powerful tool for the detection of dengue virus.
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