In order to explore the killing effect of 222 nm excimolecular lamp on microorganisms under laboratory conditions,this study used the bacterial propagules of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)as the indicator bacteria to investigate the disinfection effect of 222 nm excimer lamp on microorganisms under different conditions,and compared it with tradi-tional low-pressure mercury lamps.The results indicate that at a distance of 32 cm from the surface of the object,the peak wavelength of the excimer lamp is 222 nm,and the irradiance value is 1796 μw/cm2,while the peak wavelength of the low-pressure mercury lamp is 253.8 nm,and the irradiance value is only 484 μw/cm2.In terms of the killing effect on microorganisms,under the condition that the bacterial solution was diluted with phosphate buffer solution,with the temperature of the bacterial carrier steel plate at 20℃,and no drying conditions were applied,the killing logarithm of Staphylococcus aureuss,Escherichia coli,and Pseudomonas aeruginosa can reach 3 after 15 seconds of irradiation with a 222 nm excimer lamp.Under the condition that the bacterial solution was diluted with tryptone soy broth with the temperature of the bacterial carrier steel plate at 20℃,4℃and-20℃,and drying conditions were applied,it took 60 s for 222 nm excimer lamp to achieve disinfection effect,and 20 min for low-pressure mercury lamp.This study shows that under the same conditions,the time required for the 222nm excimer lamp to achieve disinfection effect is much shorter than that of the low-pressure mercury lamp,and the irradiance value and bactericidal effect of the 222 nm excimer lamp are stronger than those of the low-pressure mercury lamp.So the 222 nm excimer lamp can be applied to the surface disinfection of cold chain goods.
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