摘要
本研究以ITS2基因为分子标记设计杂交探针,制作DNA芯片鉴定杂鳞库蚊复合组、白纹伊蚊亚组和尖音库蚊复合组中的8种/亚种蚊虫.结果表明,以ITS2基因为分子标记设计的DNA芯片可用于3个复合组(亚组)组间蚊虫以及杂鳞库蚊复合组的组内蚊虫鉴定,且白纹伊蚊亚组探针、尖音库蚊复合组探针、杂鳞库蚊复合组探针、三带喙库蚊探针、伪杂鳞库蚊探针、致倦库蚊探针、尖音库蚊探针具有良好的专一性和特异性.现场测试表明,该DNA芯片可应用于识别不同发育阶段和不同地区的同种蚊虫,但对尖音库蚊和致倦库蚊表现出非专一性杂合.
Abstract
This study employed the ITS2 gene as a molecular marker for the design of hybridization probes,creating a DNA chip for the identification of eight species/subspecies within the Cules vishnui complex,Aedes albopictus subgroup,and Cx.pipiens com-plex.The findings indicate that the DNA chip,developed with the ITS2 gene as the molecular marker,is suitable for the inter-complex(subgroup)and intra-complex identification of mosquitoes,including those within the Cx.vishnui complex.The probes specific to the Ae.albopictus subgroup,Cx.pipiens complex,Cx.vishnui complex,Cx.tritaeniorhynchus,Cx.pseudovishnui,Cx.quinquefasciatus,and Cx.pipiens displayed high specificity and selectivity.Field assessments have confirmed the applicability of the DNA chip in iden-tifying conspecific mosquitoes at different developmental stages and from diverse regions.However,the chip exhibited non-specific hybridization for Cx.pipiens and Cx.quinquefasciatus.
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