Research on Escherichia coli DNA Analysis Based on Dual-target Triggered Hybridization Chain Reaction
This study establishes a novel method for detecting Escherichia coli nucleic acids using a dual-target triggered hybridization chain reaction(HCR).By targeting two specific nucleic acid sequences from the gene of Escherichia coli O157:H7,hairpin probes labeled with FAM fluorescent group and quencher group were designed.The target sequences were amplified through HCR and reaction conditions were optimized.Results indicated that under the condition of 0.15 mol/L Na+in 0.02 mol/L HEPES buffer,the method exhibited good stability and selectivity,with no interference signals from Helicobacter pylori,Shigella,Staphylococcus aureus,or Salmonella.The detection method showed a good linear relationship within the concentration range of 0.001 to 20 nmol/L,with a detection limit of 0.001 nmol/L.In actual sample testing,this method accurately distinguished true positive samples,offering a promising new approach for Escherichia coli detection.
NETL
NSTL
万方数据
