首页|MALDI-TOF MS法快速鉴定江苏口岸输入性蜚蠊携带致病菌

MALDI-TOF MS法快速鉴定江苏口岸输入性蜚蠊携带致病菌

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本文旨在探索利用飞行时间质谱MALDI-TOF MS快速鉴定江苏口岸输入性病媒生物蜚蠊携带致病菌种类特征,为防控蜚蠊携带致病菌提供技术指导.通过将口岸截获的输入性病媒生物蜚蠊组织研磨后进行细菌培养,对单一菌落分别通过基质辅助激光解吸电离飞行时间质谱(Matrix-Assisted Laser Desorption/Ionization Time of Flight,MALDI-TOF MS)法进行鉴定,以检测蜚蠊携带致病菌种类特征.结果显示,通过MALDI-TOF MS法鉴定出蜚蠊携带的菌群主要是革兰阴性杆菌,包括肺炎克雷伯菌(Klebsiella pneumoniae,KPN)、产酸克雷伯菌(Klebsiella oxytoca,Kox)、铜绿假单胞菌(Pseudomonas aeruginosa,PAE)、大肠埃希菌(Escherichia coli,E.coli)、奇异变形杆菌(Proteus mirabilis)及其亚型等.少数为携带革兰阳性菌,包括金黄色葡萄球菌(Staphylococcus aureus,MRSA)及其他革兰阳性葡萄球菌的亚型、蜡样芽孢杆菌(Bacillus cereus)及其他芽孢杆菌等.综上,MALDI-TOF-MS法可实现对口岸输入性病媒生物蜚蠊携带致病菌的快速鉴定,为口岸输入性蜚蠊携带传播疾病的防控提供技术支持.
Rapid Identification of Pathogenic Bacteria Carried by Imported Cockroaches at Jiangsu port Using MALDI-TOF MS Method
This study aims to explore the rapid identification of the pathogenic bacteria carried by cockroaches,an imported vec-tor species at Jiangsu ports,using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS),to provide technical guidance for the prevention and control of cockroaches.By grinding the tissues of intercepted imported vector cockroaches and culturing the bacteria,we identified individual colonies using MALDI-TOF MS to detect the species characteristics of pathogenic bacteria carried by cockroaches.According to the result of MALDI-TOF MS,the main bacteria carried by cockroaches were Gram-negative bacilli,including Klebsiella pneumoniae(KPN),Klebsiella oxytoca(Kox),Pseudomonas aeruginosa(PAE),Escherichia coli(E.coli),Proteus mirabilis and their subtypes.A minority were Gram-positive bacteria,including Staphylococcus aureus(MRSA)and other Gram-positive staphylococcus subtypes,Bacillus cereus and other bacillus species.These findings indicate that MALDI-TOF-MS method can achieve rapid identification of pathogenic bacteria carried by imported vector-borne cockroaches at ports,and provide technical support for the prevention and control of imported vector-borne diseases.

Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS)cockroachimportedpathogenic bacteria

孟媛、苏小建、杨国平、蔡丽萍、张荣、易海华、田锋、耿合员

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江苏国际旅行卫生保健中心(南京海关口岸门诊部)南京 210019

常熟海关综合技术服务中心 常熟 215500

新疆国际旅行卫生保健中心(乌鲁木齐海关口岸门诊部)乌鲁木齐 830011

基质辅助激光解吸电离飞行时间质谱 蜚蠊 口岸输入 致病菌

国家重点研发计划海关总署科研项目南京海关科研项目南京海关科研项目

2021YFC23012002023HK032022KJ152023KJ02

2024

中国口岸科学技术
中国质检报刊社

中国口岸科学技术

影响因子:0.051
ISSN:1002-4689
年,卷(期):2024.6(8)
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