Electrochemical Sensor for the Determination of Histamine in Food by Molecular Imprinting-Ru(bpy)32+Probe System
Histamine(HA)is an important biochemical substance in the human body,but excessive consumption of HA can lead to problems such as histamine intolerance and histamine poisoning.Therefore,it is particularly necessary to develop selective and low-cost detection methods for HA in food.In this study,an HA molecular imprinting sensor was prepared on the surface of a glassy carbon electrode using HA as a template molecule and o-phenylenediamine as a functional monomer through electrochemical polymerization.The sensor used tris(2-pyridyl)ruthenium(Ru(bpy)32+)as a probe,and based on the quantitative relationship between histamine concentration and current after adsorption and elution of histamine molecules on the sensor,the content of histamine in several food substrates was determined by cyclic voltammetry characteristic curve.The detection linear concentration range of the prepared sensor was 1.0×10-5 to 1.0×10-7 mol/L,with a correlation coefficient of 0.993 and a detection limit of 8.4×10-8 mol/L(3 σ/S).The recovery rate of spiked samples was between 91.2%and 106.6%,with an RSD value of less than 5%.This method is simple to operate,has good specificity,and can be applied to the detection of HA in different food substrates.
NETL
NSTL
万方数据
