Study of Nucleic Acid Aerosol Scavenger Performance Evaluation Method
Nucleic acid testing based on Polymerase Chain Reaction(PCR)technology is widely used in vitro diagnostics,such as human and animal disease screening,due to its excellent specificity,sensitivity,and relatively convenient operation.However,even extremely small amounts of nucleic acid contamination may lead to false-positive problems,thereby compromising test accuracy.Thorough removal of residual nucleic acid fragments from the experimental environment can effectively reduce the occurrence of false-positive test results.This study proposes a validation and comprehensive evaluation scheme for nucleic acid aerosol pollution removal devices.By artificially simulating nucleic acid aerosol contamination,this study employed Real-Time Quantitative Polymer Chain Reaction(RT-PCR)technology to detect the differences in Cycle Threshold(Ct)values before and after the use of a commercially available nucleic acid aerosol clearance instrument.The results indicated that Ct values significantly decreased after 0.5 hours,1 hour,and overnight clearance,suggesting that the instrument meets laboratory requirements for nucleic acid clearance.Notably,the Ct value reached 40.00 or higher after overnight clearance,indicating that overnight clearance can more effectively eliminate airborne aerosols.This study provides a technical method for the performance verification and comprehensive evaluation of nucleic acid aerosol removal devices,and provides a solution for daily nucleic acid contamination removal in molecular testing laboratories.
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