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探求适用莱士曼氏乳酸杆菌传代培养方法

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本研究针对传统的15 d传代方法,改用7 d石蜡保存法和甘油肉汤保存法,探索适合莱士曼氏乳酸杆菌传代培养方法.通过长期实验验证,分析标准曲线和相对标准偏差(Relative Standard Deviation,RSD),得到这两种方法标准曲线线性关系r2 均>0.99,相对标准偏差RSD分别为 2.5%~3.7%和 2.9%~7.2%.证实了用 7 d石蜡保存法、甘油肉汤保存法这两种方法保存的莱士曼氏乳酸杆菌活性稳定,活化周期短,可满足维生素B12 检测要求.
Study on Suitable Subculture Methods of Lactobacillus leishmannii
In this study,the traditional method of 15-day passage method was changed to 7-day passage method with liquid paraffin and glycerin broth preservation method to explore a suitable subculture method of Lactobacillus leishmannii.The standard curve and the relative standard deviation(RSD)of these two methods were analyzed through long-term experimental verification.The results showed that these two methods standard curve linear relationship r2 was>0.99,and the RSD were 2.5%-3.7%and 2.9%-7.2%,respectively.This study proved that Lactobacillus leishmannii saved by the 7-day passage method with liquid paraffin and the glycerin broth preservation method had stable activity and short activation period,which can meet the requirement of vitamin B12 detection.

microbial methodvitamin B12Lactobacillus leishmanniipreservation method

吴巧维、王潇伦、金园园、周丹虹、袁天虎、劳华均

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宁波中盛产品检测有限公司 宁波 315012

宁波海关技术中心 宁波 315012

微生物法 维生素B12 莱士曼氏乳酸杆菌 保存方法

宁波中盛产品检测有限公司科研项目

2022ZS003

2024

中国口岸科学技术
中国质检报刊社

中国口岸科学技术

影响因子:0.051
ISSN:1002-4689
年,卷(期):2024.6(9)
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