探求适用莱士曼氏乳酸杆菌传代培养方法
Study on Suitable Subculture Methods of Lactobacillus leishmannii
吴巧维 1王潇伦 1金园园 1周丹虹 1袁天虎 1劳华均2
作者信息
- 1. 宁波中盛产品检测有限公司 宁波 315012
- 2. 宁波海关技术中心 宁波 315012
- 折叠
摘要
本研究针对传统的15 d传代方法,改用7 d石蜡保存法和甘油肉汤保存法,探索适合莱士曼氏乳酸杆菌传代培养方法.通过长期实验验证,分析标准曲线和相对标准偏差(Relative Standard Deviation,RSD),得到这两种方法标准曲线线性关系r2 均＞0.99,相对标准偏差RSD分别为 2.5%～3.7%和 2.9%～7.2%.证实了用 7 d石蜡保存法、甘油肉汤保存法这两种方法保存的莱士曼氏乳酸杆菌活性稳定,活化周期短,可满足维生素B12 检测要求.
Abstract
In this study,the traditional method of 15-day passage method was changed to 7-day passage method with liquid paraffin and glycerin broth preservation method to explore a suitable subculture method of Lactobacillus leishmannii.The standard curve and the relative standard deviation(RSD)of these two methods were analyzed through long-term experimental verification.The results showed that these two methods standard curve linear relationship r2 was＞0.99,and the RSD were 2.5%-3.7%and 2.9%-7.2%,respectively.This study proved that Lactobacillus leishmannii saved by the 7-day passage method with liquid paraffin and the glycerin broth preservation method had stable activity and short activation period,which can meet the requirement of vitamin B12 detection.
关键词
微生物法/维生素B12/莱士曼氏乳酸杆菌/保存方法Key words
microbial method/vitamin B12/Lactobacillus leishmannii/preservation method引用本文复制引用
基金项目
宁波中盛产品检测有限公司科研项目(2022ZS003)
出版年
2024
NETL
NSTL
万方数据