基于UHPLC-MS/MS的红肉猕猴桃中花色素检测方法研究
Development of a UHPLC-MS/MS Method for the Detection of Anthocyanins in Red Flesh Kiwifruit
董莹 1陈子凡 2王国胜 3池少铃 4谢珊珊 5郑少锋 1张南峰1
作者信息
- 1. 广东省口岸安全智能化检测重点实验室 广州 510700;黄埔海关技术中心 东莞 523000
- 2. 河源市食品检验所 河源 517000
- 3. 河源海关技术中心 河源 517000
- 4. 河源市药品检验所 河源 517000
- 5. 广州采芝林药业有限公司 广州 510000
- 折叠
摘要
本文建立了一种基于超高效液相色谱-质谱联用法(Ultra High-Performance Liquid Chromatography-Mass Spectrometry,UHPLC-MS/MS)测定红肉猕猴桃中矢车菊素、飞燕草素、矮牵牛素、花葵素、芍药素、锦葵色素的方法.样品经含0.1%甲酸的甲醇水溶液直接提取,在电喷雾电离源(Electron Spray Ionization,ESI)于正离子模式下,采用选择性反应监测(Selected Reaction Monitoring,SRM)进行检测.结果显示,在5～500 ng/mL含量之间质量浓度范围内具有良好线性关系,线性相关系数r均在 0.997 以上,回收率为 65.0%～99.6%,精密度为 4.1%～9.1%,检出限为 0.0003 mg/kg,定量限为 0.001 mg/kg.结果表明,该方法可对红肉猕猴桃中6 种花色素物质进行含量检测,具有简便高效、结果准确、灵敏度高、重复性好的特点.
Abstract
This study established a method for the determination of cyanidin,delphinidin,petunidin,malvidin,peonidin,and pelargonidin in red flesh kiwifruit using ultra high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Samples were directly extracted with a methanol aqueous solution containing 0.1%formic acid and analyzed using electrospray ionization(ESI)in positive ion mode with selected reaction monitoring(SRM).The results showed good linearity within the concentration range of 5-500 ng/mL,with linear correlation coefficients(r)above 0.997.The recovery rates were between 65.0%and 99.6%,precision ranged from 4.1%to 9.1%,and the limits of detection and quantification were 0.0003 mg/kg and 0.001 mg/kg,respectively.The method demonstrated its capability for the content determination of six anthocyanin compounds in red flesh kiwifruit,characterized by simplicity,efficiency,accuracy,high sensitivity,and good repeatability.
关键词
红肉猕猴桃/花色素/超高效液相色谱-质谱Key words
red kiwi/anthocyanins/ultra high-performance liquid chromatography-mass spectrometry(UHPLC-MS/MS)引用本文复制引用
基金项目
广东省口岸安全智能化检测重点实验室项目(2023B1212010011)
河源市社会发展科技计划项目(230613111602379)
出版年
2024
NETL
NSTL
万方数据