Study on the Impact of FTA Cards on Preserving Respiratory Samples
This paper evaluated the practical application of FTA(Flinders Technology Associates)cards in respiratory tract sample preservation.40 respiratory tract samples were collected with conventional oropharyngeal swab tubes and FTA cards respectively.Quantitative real-time polymerase chain reaction(qPCR)was used to detect related respiratory pathogens.The FTA cards were stored at 4℃,normal temperature(20℃),37℃for 72 h,168 h,336 h,672 h,and the Cycling threshold(Ct)values of pathogen nucleic acid under different environmental conditions were compared.12 patients were found to be positive for respiratory tract related pathogens.The results of FTA card samples were consistent with those of oropharyngeal swabs,and the difference was not statistically significant.For Streptococcus pneumoniae and Haemophilus influenzae samples,compared with those from samples not preserved on FTA cards,the Ct values were significantly increased when stored at 4℃and normal temperature for more than 672 hours,and when stored at 37℃for more than 168 hours.For rhinovirus,coronavirus-229E,metapneumovirus and influenza A virus,compared with those from samples not preserved on FTA cards,the Ct values increased significantly when stored at 4℃for 336 h,168 h at room temperature,and 72 h at 37℃.For rhinovirus,coronavirus-229E and metapneumovirus,no positive signal was detected after storage at 37℃for 672h.As a respiratory tract sample collection method,FTA cards can be stored at room temperature for 336 h without affecting the pathogen qPCR results,but the storage temperature should be reduced as much as possible to shorten the storage time,so as not to affect the subsequent experimental results.
NETL
NSTL
万方数据
