水产养殖环境中多种农药残留检测方法研究
Study on Detection Methods for Multiple Pesticide Residues in Aquaculture Environment
张柏瑀 1董鸿竺 2杜姗姗 2张彤 2万建春 3姜莉 2耿庆华 2刘瑜2
作者信息
- 1. 沈阳市和平区疾病预防控制中心 沈阳 110005
- 2. 沈阳海关技术中心 沈阳 110016
- 3. 南昌海关技术中心 南昌 330000
- 折叠
摘要
本研究采用改进的QuEChERS前处理技术与超高效液相色谱-串联质谱仪,建立了水产养殖环境中32种农药同时测定的分析方法.试样经乙腈提取,改进的QuEChERS净化,采用C18 色谱柱分离,流动相为乙腈+甲酸水,质谱扫描模式为多反应监测(MRM),外标法定量.结果显示,该方法的检出限为 0.003～0.015 mg/L,定量限为 0.01～0.05 mg/L,回收率为75%～113%,相对标准偏差为0.132%～6.73%.结果表明,该方法简便、快速、准确,适用于水产养殖环境中32种农药的同时检测.
Abstract
s This study developed an analytical method for the simultaneous determination of 32 pesticides in aquaculture environments using an improved QuEChERS pre-treatment technology coupled with ultra-high performance liquid chroma-tography-tandem mass spectrometry by optimizing pre-treatment and instrument conditions.The sample was extracted with acetonitrile,purified with improved QuEChERS method,and separated using a C18 chromatographic column.The mobile phase consisted of acetonitrile and formic acid water,with mass spectrometry scanning mode operated in multi reaction monitoring(MRM).Quantification was performed using the external standard method.The detection limit of this method ranged from 0.003 to 0.015 mg/L,with a quantification limits from 0.01-0.05 mg/L,recovery rate 75%-113%,relative standard deviation 0.132%-6.73%.This method is simple,fast,and accurate,making it suitable for the simultaneous detection of 32 pesticides in aquaculture environments.
关键词
农药/液相色谱-串联质谱/水产养殖环境/改进的QuEChERSKey words
pesticides/UPLC-MS/MS/aquaculture environments/improved QuEChERS引用本文复制引用
出版年
2024
NETL
NSTL
万方数据