CRISPR-Cas technology,a powerful nucleic acid detection technology based on the principles of gene editing,has gained broad application prospect in the field of nucleic acid detedction due to its distinctive advantages of high specificity,high sensitivity,fast and convenient,and wide applicability.In recent years,the application of this technology in nucleic acid detection has made rapid development,and has been used to detect viruses,bacteria,fungi,gene mutations and other nucleic acid targets,and has shown better performance than traditional methods.This article overviews the application of CRISPR-Cas technology in nucleic acid detection,encompassing detection methods for targeting DNA,RNA,and DNA/RNA.Furthermore,a detailed discussion on the advantages and limitations of CRISPR-Cas technology is presented.Notably,in terms of application,the rapid,convenient,and sensitive features of CRISPR-Cas technology render it a promising tool for rapid screening and on-site detection in port inspection and quarantine,potentially serving as a new weapon in the fight against biological threats at ports.
CRISPR-Cas technologynucleic acid detectionspecificitysensitivityport inspection and quarantine
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