首页|Genetic differentiation of Helicoverpa armigera (Hübner) and H.Assulta (Guenée) (Lepidoptera: Noctuidae) based on AFLP markers

Genetic differentiation of Helicoverpa armigera (Hübner) and H.Assulta (Guenée) (Lepidoptera: Noctuidae) based on AFLP markers

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Here we use amplified fragment length polymorphism (AFLP) to assess genetic differentiation of Helicoverpa armigera and H. assulta. The results indicated that both species-specific fingerprints and cluster analysis showed the ability of AFLP technique to discriminate the two sibling species; among a total 1963 AFLP markers amplified from nine primer combinations: 777 (39.6%) were H. armigera-specific,602 (30.7%) were H. assultaspecific,and 584 (29.7%) were common bands. The mean number of H. armigera-specific bands was significantly more than that of H. assulta-specific bands for nine primer combinations (P＜0.05); the intraspecific distance of H. armigera and H. assulta was 0.123 0 and 0.110 7 respectively,and the interspecific distance was 0.178 3. In addition,the percentage of polymorphic loci and estimated average heterozygosity were used to estimate genetic diversity of the two species. This study therefore demonstrates that AFLP analysis is a sensitive and reliable technique to study genetic differentiation and genetic relationships between species and provides sufficient molecular markers for future linkage map construction,location and eventual cloning of genes involved in traits differentiation.

AFLP analysisgenetic differentiationHelicoverpa armigeraH. assultamolecular markers

QING-LEI MING、CHEN-ZHU WANG

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State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Xuzhou Normal University,Xuzhou,Jiangsu,China

Graduate School of the Chinese Academy of Sciences,Beijing Xuzhou Normal University,Xuzhou,Jiangsu,China

School of Life Sciences,Xuzhou Normal University,Xuzhou,Jiangsu,China

国家自然科学基金国家自然科学基金

3033010030471148

2006

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2006.13(6)
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