首页|Genome-wide analysis of odorant-binding proteins and chemosensory proteins in the sweet potato whitefly,Bemisia tabaci

Genome-wide analysis of odorant-binding proteins and chemosensory proteins in the sweet potato whitefly,Bemisia tabaci

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Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) of insects are thought to play roles in olfactory recognition affecting host choice,copulation,reproduction and other behaviors.Previous descriptions of OBPs and CSPs in the whitefly Bemisia tabaci often provided no or incomplete genetic information.In this study,we present a genome-wide and transcriptome-wide investigation of the OBPs and CSPs in B.tabaci MEAM1 (Middle East-Asia Minor1 species).Eight OBP and 19 CSP genes were identified that covered all previous sequences.Phylogenetic analyses showed that the CSP genes had a lineage-specific expansion (BtabBCSP1,BtabBCSP3,BtabBCSP13,BtabBCSP17,BtabBCSP18 and BtabBCSP19).Expression profiling of OBPs and CSPs by transcriptome sequencing and quantitative real-time polymerase chain reaction (qPCR) revealed that expression patterns differed among developmental stages ofB.tabaci MEAM 1.Five OBP genes and 11 CSP genes significantly differed between males and females;four of the 19 CSP genes were highly expressed in adults,while two were highly expressed in nymphs.The expression profiles of the OBP and CSP genes in different tissues of B.tabaci MEAM1 adults were analyzed by qPCR.Four OBP genes found in B.tabaci MEAM1 were highly expressed in the head.Conversely,only two CSPs were enriched in the head,while the other six CSPs were specifically expressed in other tissues.Our results provide a foundation for future research on OBPs and CSPs in B.tabaci.

Bemisia tabaciCSPsexpression patternsgenome-wide identificationOBPsphylogenetic

Yang Zeng、Yu-Ting Yang、Qing-Jun Wu、Shao-Li Wang、Wen Xie、You-Jun Zhang

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Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China

Department of Agriculture of Yangtze University, Jingzhou, Hubei Province, China

This research was supported by the National Natural Science Foundation of ChinaThis research was supported by the National Natural Science Foundation of ChinaThis research was supported by the National Natural Science Foundation of ChinaBeijing Nova ProgramScience and Technology Innovation Project from the Chinese Academy of Agricultural SciencesScience and Technology Innovation Project from the Chinese Academy of Agricultural SciencesBeijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables.Performed the analysis:YZ,WX,YJZ,YTY,SLW,Q

314201039193167203231772172Z171100001117039CAAS-XTCX2016017002

2019

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCDCSCDSCI
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2019.26(4)
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