Identification and functional characterization of female antennae-biased odorant receptor 23 involved in acetophenone detection of the Indian meal moth Plodia interpunctella

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外文摘要：The Indian meal moth,Plodia interpunctella(Lepidoptera:Pyralidae),a globally distributed storage pest,relies on odors that are emitted from stored foods to select a suitable substrate for oviposition.However,the molecular mechanism underly-ing the chemical communication between P interpunctella and its host remains elusive.In this study,130 chemosensory genes were identified from the transcriptomes of 7 P.interpunctella tissues,and the quantitative expression levels of all 56 P interpunctella odorant receptor genes(PintORs)were validated using real-time quantitative polymerase chain reaction.The functional characteristics of 5 PintORs with female antennae-biased expression were investigated using 2-electrode voltage clamp recordings in Xenopus lae-vis oocytes.PintOR23 was found to be specifically tuned to acetophenone.Acetophenone could elicit a significant electrophysiological response and only attracted mated females when compared with males and virgin females.In addition,molecular docking predicted that the hydrogen bonding sites,TRP-335 and ALA-167,might play key roles in the bind-ing of PintOR23 to acetophenone.Our study provides valuable insights into the olfac-tory mechanism of oviposition substrate detection and localization in P interpunctella and points toward the possibility of developing eco-friendly odorant agents to control pests of stored products.

外文关键词：

acetophenonedockingEAGodorant receptorPlodia interpunctellaTEVC

作者：

Qi Chen、Xiaoyan Zhu、Guoqing Kang、Qiling Yu、Qingxin Liu、Lin Du、Yi Yang、Xinyu He、Ying Zhao、Junjie Zhang、Ying Hu、Bingzhong Ren

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作者单位：

Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization,School of Life Sciences,Northeast Normal University,Changchun,China

Key Laboratory of Vegetation Ecology,Ministry of Education,Northeast Normal University,Changchun,China

Jilin Provincial Engineering Laboratory of Avian Ecology and Conservation Genetics,Northeast Normal University,Changchun,China

Engineering Research Center of Natural Enemies,Institute of Biological Control,Jilin Agricultural University,Changchun,China

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基金：

China Postdoctoral Science FoundationFundamental Research Funds for the Central UniversitiesYoung Science and Technology Talent Support Project of Jilin Province

项目编号：

2021M690588135111010QT202121

出版年：

2024

DOI：

10.1111/1744-7917.13220

中国昆虫科学(英文版)

中国昆虫学会中科院动物所

中国昆虫科学(英文版)

CSTPCD

影响因子：0.484

ISSN：1672-9609

年,卷(期)：2024.31(1)

参考文献量57