首页|MicroR-9c-5p and novel-mir50 co-target Akt to regulate Lasioderma serricorne reproduction

MicroR-9c-5p and novel-mir50 co-target Akt to regulate Lasioderma serricorne reproduction

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High fecundity is a common characteristic of insect pests which increases the difficulty of population control.Serine/threonine kinase Akt is an indispensable compo-nent of the insulin signaling pathway.Silencing of LsAkt severely hinders reproduction in Lasioderma serricorne,a stored product insect pest.However,the post-transcriptional pathway of LsAkt in L.serricorne remains unknown.This study identified 2 binding sites of miR-9c-5p and novel-mir50 in the coding sequences ofLsAkt.The expression profiles of 2 microRNAs(miRNAs)and LsAkt displayed an opposite pattern during the adult stages.Luciferase reporter assay showed that novel-mir50 and miR-9c-5p could downregulate the expression of LsAkt.Overexpression of miR-9c-5p and novel-mir50 by injection of mimics inhibited the expression of LsAkt and reduced oviposition,decreased egg hatchability,and blocked ovarian development.It also decreased the expression of genes involved in ovar-ian development(LsVg and LsVgR)and the nutritional signaling pathway(LsTOR,LsS6K,and Ls4EBP),and reduced the phosphorylation of Akt.Conversely,injection of miR-9c-5p and novel-mir50 inhibitors induced the expressions of LsAkt,LsVg,LsVgR,LsTOR,LsS6K,and Ls4EBP,enhanced Akt phosphorylation level,and accelerated ovarian devel-opment.Injection of bovine insulin downregulated the expression of miR-9c-5p and novel-mir50 and upregulated the LsAkt expression.It also rescued the reproductive development defects associated with miR-9c-5p/novel-mir50 overexpression,forming a positive regula-tory loop of insulin signaling.These results indicate that miR-9c-5p/novel-mir50 regulates the female reproduction of L.serricorne by targeting Akt in response to insulin signaling.The data also demonstrate the effects of the insulin/miRNA/Akt regulatory axis in insect reproduction.

AktinsulinLasioderma serricornemicroRNAreproduction

Yi Yan、Dong-Dong Qin、Hong Yang、Kang-Kang Xu、Can Li、Wen-Jia Yang

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Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region,Institute of Entomology,Guizhou University,Guiyang,China

Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region,College of Biology and Environmental Engineering,Guiyang University,Guiyang,China

National Natural Science Foundation of ChinaProject of China Tobacco Guizhou Industrial Co.,LtdSpecial Project for Local(Guizhou)Science and Technology Development under the guidance of the Central GovernmentGraduate Research Project of Guizhou ProvinceProgram of Excellent Innovation Talents in Guizhou Province

321606372017-25QKZYD[2022]4013QJHYJSKYJJ[2021]19420206003

2024

10.1111/1744-7917.13221

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(1)
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