首页|Odorant-binding protein 19 in Monochamus alternatus involved in the recognition of a volatile strongly emitted from ovipositing host pines
Odorant-binding protein 19 in Monochamus alternatus involved in the recognition of a volatile strongly emitted from ovipositing host pines
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Monochamus alternatus is the primary carrier of pine wood nematodes,which pose a serious threat to Pinus spp.in many countries.Newly emerging M.alternatus adults feed on heathy host pines,while matured adults transfer to stressed host pines for mat-ing and oviposition.Several odorant-binding proteins(OBPs)of M.alternatus have been proved to aid in the complex process of host location.To clarify the corresponding rela-tions between OBPs and pine volatiles,more OBPs need to be studied.In this research,MaltOBP19 showed a specific expression in the antennae and mouthparts of M.alterna-tus,and it was marked in 4 types of antenna sensilla by immunolocalization.Fluorescence binding assays demonstrated the high binding affinity of MaltOBP19 with camphene and myrcene in vitro.In Y-tube olfactory experiments,M.alternatus adults were attracted by camphene and RNAi of OBP19 via microinjection significantly decreased their attraction index.Myrcene induced phobotaxis,but RNAi had no significant effect on this behavior.Further,we found that ingesting dsOBP19 produced by a bacteria-expressed system with a newly constructed vector could lead to the knockdown of MaltOBP 19.These results suggest that MaltOBP 19 may play a role in the process of host conversion via the recog-nition of camphene,which has been identified to be strongly released in stressed host pines.In addition,it is proved that knockdown of OBP can be achieved by oral admin-istration of bacteria-expressed double-stranded RNA in M.alternatus adults,providing a new perspective in the control of M.alternatus.
bacteria-expressed dsRNAcampheneJapanese pine sawyer beetleMal-tOBP19RNAistressed host pines
Ruinan Yang、Dongzhen Li、Shancheng Yi、Yi Wei、Manqun Wang
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Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,China
Key Laboratory of Forest Protection of National Forestry and Grassland Ad-ministration,Ecology and Nature Conservation Institute,Chinese Academy of Forestry,Beijing,China
National Key Research and Development Program of ChinaNational Natural Science Foundation of China
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