首页|Functional characterization of Bmcap in uric acid metabolism in the silkworm

Functional characterization of Bmcap in uric acid metabolism in the silkworm

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After a millennium of domestication,numerous silkworm mutants have emerged that exhibit transparent epidermis,which is caused by abnormally low levels of uric acid.We identified the Bombyx mori gene Bmcap(BMSK0003832)as the homolog of cappuccino,a subunit of the biogenesis of lysosome-related organelles complex-1(BLOC-1)that has been extensively characterized in human,mouse,and insect species,by analyzing the amino acid sequences of putative purine metabolism genes.Using the clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated pro-tein 9 system,we disrupted Bmcap,resulting in decreased uric acid levels in the silkworm epidermis and a translucent skin phenotype.In the Bmcap mutant,the purine metabolism,nitrogen metabolism,pyrimidine metabolism,and membrane system were altered com-pared to the wild type.Biogenesis of lysosome-related organelle complex genes play a role in the pigmentation and biogenesis of lysosome-related organelles(LROs)in platelets,melanocytes,and megakaryocytes.LROs exhibit unique morphologies and functions in various tissues and cells.Investigation of the Bmcap mutant will enhance our understand-ing of the uric acid metabolic pathway in silkworms,and this mutant offers a valuable silkworm model for LRO studies.

BLOC-1 subunitBombyx moricappuccinotranslucent mutationuric acid

Linmeng Tang、Dehong Yang、Zhiwei Liu、Yaohui Wang、Xu Yang、Yujia Liu、Dongbin Chen、Zheng Tang、Yongping Huang

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Key Laboratory of Insect Developmental and Evolutionary Biology,Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai,China

CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing,China

Central Research Institute,Shanghai Pharmaceuticals Holding Co.,Ltd.,Shanghai,China

Departments of Neonatology,International Peace Maternity and Child Health Hospital of China Welfare Institution,School of Medicine,Shanghai Jiao Tong University,Shanghai,China

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National Science Foundation of ChinaNational Science Foundation of ChinaStrategic Priority Research Program of Chinese Academy of Sciences

3202100131830093XDB11010600

2024

10.1111/1744-7917.13236

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(1)
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