首页|The appearance of cytoplasmic cytochrome C precedes apoptosis during Drosophila salivary gland degradation

The appearance of cytoplasmic cytochrome C precedes apoptosis during Drosophila salivary gland degradation

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Apoptosis is an important process for organism development that functions to eliminate cell damage,maintain homeostasis,and remove obsolete tissues during morpho-genesis.In mammals,apoptosis is accompanied by the release of cytochrome C(Cyt-c)from mitochondria to the cytoplasm.However,whether this process is conserved in the fruit fly,Drosophila melanogaster,remains controversial.In this study,we discovered that during the degradation of Drosophila salivary gland,the transcription of mitochondria apoptosis factors(MAPFs),Cyt-c,and death-associated APAF1-related killer(Dark)en-coding genes are all upregulated antecedent to initiator and effector caspases encoding genes.The proteins Cyt-c and the active caspase 3 appear gradually in the cytoplasm dur-ing salivary gland degradation.Meanwhile,the Cyt-c protein colocates with mito-GFP,the marker indicating cytoplasmic mitochondria,and the change in mitochondrial membrane potential coincides with the appearance of Cyt-c in the cytoplasm.Moreover,impeding or promoting 20E-induced transcription factor E93 suppresses or enhances the staining of Cyt-c and the active caspase 3 in the cytoplasm of salivary gland,and accordingly de-creases or increases the mitochondrial membrane potential,respectively.Our research pro-vides evidence that cytoplasmic Cyt-c appears before apoptosis during Drosophila salivary gland degradation,shedding light on partial conserved mechanism in apoptosis between insects and mammals.

active caspase 3apoptosisCyt-cDrosophilasalivary gland

Shihui Long、Wenxin Cao、Yongyu Qiu、Ruohan Deng、Jiali Liu、Lidan Zhang、Renke Dong、Fengxin Liu、Sheng Li、Haigang Zhao、Na Li、Kang Li

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Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Institute of Insect Science and Technol-ogy,School of Life Sciences,South China Normal University,Guangzhou,China

Guangdong Laboratory for Lingnan Modern Agriculture,South China Agricultural University,Guangzhou,China

Guangmeiyuan R&D Center,Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,South China Normal University,Meizhou,Guangdong Province,China

School of Food Science and Engineering,South China University of Technology,Guangzhou,China

ChemPartner PharmaTech Co.,Ltd,Jiangmen,Guangdong Province,China

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Laboratory of Lingnan Modern Agriculture ProjectNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Science Foundation of Guangdong ProvinceNational Science Foundation of Guangdong Province

NT202100332070491319003552022A15150104572022A1515011759

2024

10.1111/1744-7917.13240

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(1)
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