首页|Characterization of the pheromone receptors in Mythimna loreyi reveals the differentiation of sex pheromone recognition in Mythimna species

Characterization of the pheromone receptors in Mythimna loreyi reveals the differentiation of sex pheromone recognition in Mythimna species

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Pheromone receptors(PRs)are key proteins in the molecular mechanism of pheromone recognition,and exploring the functional differentiation of PRs between closely related species helps to understand the evolution of moth mating systems.Pheromone components of the agricultural pest Mythimna loreyi have turned into(Z)-9-tetradecen-l-yl acetate(Z9-14:OAc),(Z)-7-dodecen-l-yl acetate(Z7-12:OAc),and(Z)-11-hexadecen-l-yl acetate,while the composition differs from that of M.separata in the genus Mythimna.To understand the molecular mechanism of pheromone recognition,we sequenced and analyzed antennal transcriptomes to identify 62 odorant receptor(OR)genes.The expression levels of all putative ORs were analyzed using differentially ex-pressed gene analysis.Six candidate PRs were quantified and functionally characterized in the Xenopus oocytes system.MlorPR6 and MlorPR3 were determined to be the recep-tors of major and minor components Z9-14:OAc and Z7-12:OAc.MlorPR1 and female antennae(FA)-biased MlorPR5 both possessed the ability to detect pheromones of sym-patric species,including(Z,E)-9,12-tetradecadien-l-ol,(Z)-9-tetradecen-l-ol,and(Z)-9-tetradecenal.Based on the comparison of PR functions between M.loreyi and M.sep-arata,we analyzed the differentiation of pheromone recognition mechanisms during the evolution of the mating systems of 2 Mythimna species.

antennal transcriptomeMythimna loreyipheromone receptorsex pheromoneXenopus oocyte

Chan Wang、Lei Liu、Tian-Yu Huang、Yu Zhang、Yang Liu、Gui-Rong Wang

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State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China

School of Forestry,Northeast Forestry University,Harbin,China

Key Laboratory of Biohazard Monitoring,Green Prevention and Control for Artificial Grassland,Ministry of Agriculture and Rural Affairs,Institute of Grassland Research of Chinese Academy of Agricultural Sciences,Inner Mongolia Hohhot,China

Guangdong Laboratory of Lingnan Modern Agriculture,Shenzhen

Genome Analysis Laboratory of the Ministry of Agriculture

Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,China

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National Natural science Foundation of ChinaNational Natural science Foundation of ChinaNational Natural science Foundation of ChinaShenzhen Science and Technology ProgramProjects subsidized by Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New DistricAgricultural Science and Technology Innovation Program(ASTIP)

321300893172502332072509KQTD20180411143628272PT202101-02

2024

10.1111/1744-7917.13215

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(1)
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