首页|Variation in Bombyx mori immune response against fungal pathogen Beauveria bassiana with variability in cell wall β-1,3-glucan

Variation in Bombyx mori immune response against fungal pathogen Beauveria bassiana with variability in cell wall β-1,3-glucan

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Entomopathogenic fungi are protected by a cell wall with dynamic structure for adapting to various environmental conditions.β-1,3-Glucan recognition proteins activate the innate immune system of insects by recognizing surface molecules of fungi.However,the associations between pathogenicity and the different components of entomopathogenic fungal cell walls remain unclear.Three Beauveria bassiana strains were selected that have significantly differing virulence against Bombyx mori.The molecular mechanisms under-lying the immune response in B.mori were investigated using RNA sequencing,which revealed differences in the immune response to different B.bassiana strains at 12 h post-infection.Immunofluorescence assays revealed that β-1,3-glucan content had an opposite trend to that of fungal virulence.β-1,3-Glucan injection upregulated BmβGRP4 expres-sion and significantly reduced the virulence of the high-virulence strain but not that of the medium-virulence or low-virulence strains.BmβGRP4 silencing in B.mori with RNA interference resulted in the opposite virulence pattern,indicating that the virulence of B.bassiana was affected by the cell walls'content of β-1,3-glucan,which could be recog-nized by BmβGRP4.Furthermore,interference with the gene CnA(calcineurin catalytic A subunit)involved in β-1,3-glucan synthesis eliminated differences in virulence between B.bassiana strains.These results indicate that strains of a single species of pathogenic fungi that have differing cell wall components are recognized differently by the innate immune system of B.mori.

Bombyx moriBmβGRP4innate immunityinsect fungal pathogenviru-lence

Yulong Wang、Zhen Liu、Xuebing Yin、Shihong Liu、Kai Wang、Rongjie Wan、Haoran Chen、Xinyang Li、Bo Huang

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Anhui Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei,China

National Science Foundation of ChinaNatural Science Foundation of the Anhui Higher Education InstitutionsNatural Science Foundation of the Anhui Higher Education Institutions

32102274KJ2020A0129YJS20210237

2024

10.1111/1744-7917.13209

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(1)
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