Effects of simulated gut pH environment on bacterial composition and pheromone production of Dendroctonus valens

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外文摘要：Bark beetles are an economically and ecologically important insect group,with aggregation behavior and thus host colonization success depends on pheromone-mediated communication.For some species,such as the major invasive forest pest in China,red tur-pentine beetle(Dendroctonus valens),gut microbiota participates in pheromone produc-tion by converting tree monoterpenes into pheromone products.However,how variation in gut microenvironment,such as pH,affects the gut microbial composition,and conse-quently pheromone production,is unknown.In this study,we fed wild caught D.valens with 3 different pH media(main host diet with natural pH of 4.7;a mildly acidic diet with pH 6 mimicking the beetle gut pH;and highly acidic diet with pH 4),and measured their effects on the gut pH,bacterial community and production of the main aggregation and anti-aggregation pheromone(verbenone).We further tested the verbenone production capacity of 2 gut bacterial isolates in different pH environments(pH 6 and 4).Compared to natural state or main host diet,feeding on less acidic diet(pH 6)diluted the acidity of the gut,whereas feeding on highly acidic diet(pH 4)enhanced it.Both changes in gut pH reduced the abundance of dominant bacterial genera,resulting in decreased verbenone production.Similarly,the highest pheromone conversion rate of the bacterial isolates was observed in pH mimicking the acidity in beetle gut.Taken together,these results indicate that changes in gut pH can affect gut microbiota composition and pheromone production,and may therefore have the potential to affect host colonization behavior.

外文关键词：

Dendroctonus valensgut bacteriagut pHverbenone

作者：

Qingjie Cao、Yu Zhao、Tuuli-Marjaana Koski、Huiping Li、Jianghua Sun

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作者单位：

College of Forestry,Hebei Agricultural University,Baoding,Hebei Province,China

State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing,China

College of Life Science/Hebei Basic Science Center for Biotic Interactions,Institute of Life Science and Green Development,Hebei University,Baoding,Hebei Province,China

基金：

National Natural science Foundation of ChinaNational Natural science Foundation of ChinaNational Natural science Foundation of ChinaOpen Project of the State Key Laboratory of the Institute of Zoology,Chinese Academy of SciencesProgram of the Natural Science Foundation of Hebei Province of ChinaHebei Agricultural University introduced talents scientific research project

项目编号：

321015293208810232061123002IPM2021C2019204244YJ201917

出版年：

2024

DOI：

10.1111/1744-7917.13210

中国昆虫科学(英文版)

中国昆虫学会中科院动物所

中国昆虫科学(英文版)

CSTPCD

影响因子：0.484

ISSN：1672-9609

年,卷(期)：2024.31(1)

参考文献量45