首页|Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper,Sogatella furcifera

Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper,Sogatella furcifera

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Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shells,and/or are difficult to collect and maintain outside of their normal environment.Such is the case with Sogatella fur-cifera,the white-backed planthopper(WBPH),a significant pest of Oryza sativa(rice)that oviposits inside rice stems.Egg extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice stem.To bypass these issues,we targeted embryos prior to oviposition via direct parental(DIPA)-CRISPR,in which Cas9 and single-guide RNAs(sgRNAs)for the WBPH eye pigment gene tryptophan 2,3-dioxygenase were injected into the hemocoel of adult females.Fe-males at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene-editing components.An evalua-tion of the offspring indicated that the highest G0 gene-edited efficacy(56.7％)occurred in females injected 2 d posteclosion,and that those mutations were heritably transmitted to the G1 generation.This study demonstrates the potential utility of DIPA-CRISPR for future gene-editing studies in non-model insect species and can facilitate the development of novel pest management applications.

CRISPR/Cas9DIPA-CRISPRgene editingtryptophan 2,3-dioxygenaseWBPH

Meng-Qi Zhang、Lang-Lang Gong、Ya-Qin Zhao、Yun-Feng Ma、Gui-Jun Long、Huan Guo、Xuan-Zheng Liu、J.Joe Hull、Youssef Dewer、Chao Yang、Ning-Ning Zhang、Ming He、Peng He

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National Key Laboratory of Green Pesticides,Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education-Center for R&D of Fine Chemicals of Guizhou University,Guiyang,China

Pest Management and Biocontrol Research Unit,US Arid Land Agricultural Research Center,USDA Agricultural Research Services,Maricopa,Arizona,USA

Phytotoxicity Research Department,Central Agricultural Pesticide Laboratory,Agricultural Research Center,Dokki,Giza,Egypt

Guizhou J if eng Seed Industry Limited Liability Company,Xingyi,Guizhou Province,China

Shandong Facility Horticulture Bioengineering Research Center,Weifang University of Science and Technology,Weifang,Shandong Province,China

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National Natural science Foundation of ChinaNational Natural science Foundation of ChinaScientific Research Foundation of Guizhou University of ChinaProgram of Talent Cultivation of Guizhou UniversityScience and Technology Support of Guizhou provinceProgram of Introducing Talents of Discipline to Universities of China(111 Program)Frontiers Science Center for Asymmetric Synthesis and Medicinal Molecules,Department of Education,Guizhou Province

32370527322606712017-33201905QKH20172956D20023Qianjiaohe KY 2020004

2024

10.1111/1744-7917.13286

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(4)
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