首页|Single-cell transcriptomes provide insights into expansion of glial cells in Bombyx mori
Single-cell transcriptomes provide insights into expansion of glial cells in Bombyx mori
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The diversity of cell types in the brain and how these change during differ-ent developmental stages,remains largely unknown.The life cycle of insects is short and goes through 4 distinct stages including embryonic,larval,pupal,and adult stages.During postembryonic life,the larval brain transforms into a mature adult version after metamor-phosis.The silkworm,Bombyx mori,is a lepidopteran model insect.Here,we character-ized the brain cell repertoire of larval and adult B.mori by obtaining 50 708 single-cell transcriptomes.Seventeen and 12 cell clusters from larval and adult brains were assigned based on marker genes,respectively.Identified cell types include Kenyon cells,optic lobe cells,monoaminergic neurons,surface glia,and astrocyte glia.We further assessed the cell type compositions of larval and adult brains.We found that the transition from larva to adult resulted in great expansion of glial cells.The glial cell accounted for 49.8％of adult midbrain cells.Compared to flies and ants,the mushroom body kenyon cell is insuf-ficient in B.mori,which accounts for 5.4％and 3.6％in larval and adult brains,respec-tively.Analysis of neuropeptide expression showed that the abundance and specificity of expression varied among individual neuropeptides.Intriguingly,we found that ion trans-port peptide was specifically expressed in glial cells of larval and adult brains.The cell atlas dataset provides an important resource to explore cell diversity,neural circuits and genetic profiles.
Xiaojing Liu、Zhongjie Zhang、Bo Hu、Kai Chen、Ye Yu、Hui Xiang、Anjiang Tan
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Jiangsu Key Laboratory of Sericultural Biology and Biotechnology,School of Biotechnology,Jiangsu University of Science and Technol-ogy,Zhenjiang,Jiangsu Province,China
Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture and Rural Affairs,The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang,Jiangsu Province,China
Guangzhou Key Laboratory of Insect Development Regulation and Application Research,Institute of Insect Science and Technology and School of Life Sciences,South China Normal University,Guangzhou,China
National Science Foundation of ChinaNational Science Foundation of ChinaNational Science Foundation of ChinaNatural Science Foundation of Jiangsu ProvinceNatural Science Foundation of Jiangsu ProvinceNatural Science Foundation of Jiangsu Province
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