Adenine nucleotide translocase 2(Ant2)is required for individualization of spermatogenesis of Drosophila melanogaster

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外文摘要：Successful completion of spermatogenesis is crucial for the perpetuation of the species.In Drosophila,spermatid individualization,a process involving changes in mito-chondrial structure and function is critical to produce functional mature sperm.Ant2,en-coding a mitochondrial adenine nucleotide translocase,is highly expressed in male testes and plays a role in energy metabolism in the mitochondria.However,its molecular func-tion remains unclear.Here,we identified an important role of Ant2 in spermatid individu-alization.In Ant2 knockdown testes,spermatid individualization complexes composed of F-actin cones exhibited a diffuse distribution,and mature sperms were absent in the sem-inal vesicle,thus leading to male sterility.The most striking effects in Ant2-knockdown spermatids were decrease in tubulin polyglycylation and disruption of proper mitochon-dria derivatives function.Excessive apoptotic cells were also observed inAnt2-knockdown testes.To further investigate the phenotype of Ant2 knockdown in testes at the molec-ular level,complementary transcriptome and proteome analyses were performed.At the mRNA level,868 differentially expressed genes were identified,of which 229 genes were upregulated and 639 were downregulated induced via Ant2 knockdown.iTRAQ-labeling proteome analysis revealed 350 differentially expressed proteins,of which 117 proteins were upregulated and 233 were downregulated.The expression of glutathione transferase(GstD5,GstE5,GstE8,and GstD3),proteins involved in reproduction were significantly regulated at both the mRNA and protein levels.These results indicate that Ant2 is crucial for spermatid maturation by affecting mitochondrial morphogenesis.

外文关键词：

adenine nucleotide translocaseDrosophila melanogasterindividualization complexesmitochondriaspermatogenesistubulin polyglycylation

作者：

Zhen He、Yang Fang、Fengchao Zhang、Yang Liu、Xinkai Cheng、Jiajia Wang、Dechen Li、Dengsong Chen、Fan Wu

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作者单位：

Industrial Crops Institute,Hubei Academy of Agriculture Sciences,Wuhan,China

Institute of Evolution & Marine Biodiversity,Ocean University of China,Qingdao,Shandong Province,China

基金：

China Agriculture Research System of MOF and MARAHubei Province Agricultural Science and Technology Innovation Center ProjectHubei Province Key Research and Development Project

项目编号：

CARS-18-SYZ102021-620-000-001-0092022BBA0079

出版年：

2024

DOI：

10.1111/1744-7917.13309

中国昆虫科学(英文版)

中国昆虫学会中科院动物所

中国昆虫科学(英文版)

CSTPCD

影响因子：0.484

ISSN：1672-9609

年,卷(期)：2024.31(4)

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