首页|Matrix metalloproteinase 2 degrades collagen Ⅰ to regulate ovarian development by association with an insulin-like peptide

Matrix metalloproteinase 2 degrades collagen Ⅰ to regulate ovarian development by association with an insulin-like peptide

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The ovary generally undergoes tissue remodeling during larval to pupal tran-sition,which includes membrane degeneration and ovariole growth.At the same time,the hormones produced by insects significantly change during metamorphosis.However,the regulatory mechanism for ovarian development and hormones is not fully understood in insects.Herein,we found that matrix metalloproteinase 2(MMP2)was highly expressed in the ovarian capsules and ovarioles,and the development was abnormal after knocking out MMP2 in Bombyx mori.The process of abnormal degradation of collagen I due to MMP2 deletion,which resulted in abnormal development of ovarioles and eggs,was analyzed in detail.The proteomics of ovaries in the MMP2-knock out and wild type strains showed a critically significant difference in the expression of a protein,insulin-like peptide(ILP).Additional analysis revealed significant alteration of ILP during ovarian development,and abnormal expression of ILP significantly affected ovarian development in vivo and MMP2 expression in vitro and in vivo.These results showed that MMP2 regulation of ovarian tis-sue remodeling is closely related to ILP expression.Our study provides new insights into the regulatory mechanism of MMP2 and ovarian development in B.mori.

Bombyx moricollagen Ⅰinsulin-like peptidematrix metalloproteinaseovarian development

Yi Wei、Xiao-Lin Zhou、Peng Chen、Tai-Hang Liu、Cheng Lu、Min-Hui Pan

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State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing,China

Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,Zhejiang Province,China

Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry,Southwest University,Chongqing,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNatural Science Foundation of ChongqingNatural Science Foundation of ChongqingMunicipal Graduate Student Research Innovation Project of Chongqing

3187242831872427cstc2021ycjhbgzxm0191cstc2021ycjhbgzxm0190CYB20116

2024

10.1111/1744-7917.13284

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(4)
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