首页|Odorant receptor orthologues from moths display conserved responses to cis-jasmone

Odorant receptor orthologues from moths display conserved responses to cis-jasmone

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In insects,the odorant receptor(OR)multigene family evolves by the birth-and-death evolutionary model,according to which the OR repertoire of each species has undergone specific gene gains and losses depending on their chemical environment,re-sulting in taxon-specific OR lineage radiations with different sizes in the phylogenetic trees.Despite the general divergence in the gene family across different insect orders,the ORs in moths seem to be genetically conserved across species,clustered into 23 major clades containing multiple orthologous groups with single-copy gene from each species.We hypothesized that ORs in these orthologous groups are tuned to ecologically important compounds and functionally conserved.cis-Jasmone is one of the compounds that not only primes the plant defense of neighboring receiver plants,but also functions as a behavior regulator to various insects.To test our hypothesis,using Xenopus oocyte recordings,we functionally assayed the orthologues of BmorOR56,which has been characterized as a spe-cific receptor for cis-jasmone.Our results showed highly conserved response specificity of the BmorOR56 orthologues,with all receptors within this group exclusively respond-ing to cis-jasmone.This is supported by the dN/dS analysis,showing that strong purifying selection is acting on this group.Moreover,molecular docking showed that the ligand binding pockets of BmorOR56 orthologues to cis-jasmone are similar.Taken together,our results suggest the high conservation of OR for ecologically important compounds across Heterocera.

cis-jasmoneevolutionary conservationfunctional characterizationmolec-ular dockingmothspurifying selection

Xiao-Qing Hou、Zhongqiang Jia、Dan-Dan Zhang、Guirong Wang

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Shenzhen Branch,Guangdong Laboratory for Lingnan Modem Agriculture,Synthetic Biology Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong Province,China

Department of Biology,Lund University,Lund,Sweden

State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaShenzhen Science and Technology ProgramSpecial Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New DistrictChina Postdoctoral Science Foundation

3220230732130089KQTD20180411143628272PT202101-022021M703548

2024

10.1111/1744-7917.13296

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(4)
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