首页|Identification and immune analysis of antimicrobial peptides from the cigarette beetle(Lasioderma serricorne)

Identification and immune analysis of antimicrobial peptides from the cigarette beetle(Lasioderma serricorne)

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Antimicrobial peptides(AMPs)in insects are endogenous peptides that are effector components of the innate defense system of the insect.AMPs may serve as an-timicrobial agents because of their small molecular weight and broad-spectrum antimi-crobial activity.In this study,we performed transcriptome analysis of cigarette beetle(Lasioderma serricorne)larvae,parasitized by the ectoparasitic wasp,Anisopteromalus calandrae.Several AMP genes were significantly upregulated following A.calandrae par-asitism,postulating the hypothesis that the parasitization enhanced the host's resistance against pathogenic microorganisms through the regulation of host AMP genes.Specifi-cally,3 AMP genes(LsDef1,LsDef2,and LsCole)were significantly upregulated and we studied their immune function in L.serricorne.Immune challenge and functional analysis showed that LsCole was responsible for the immune response against Gram-negative and Gram-positive bacteria,while LsDefl and LsDef2 were involved in insect defense against Gram-positive bacteria.Purified recombinant LsCole exhibited antimicrobial activities against the Gram-negative bacterium Escherichia coli and the Gram-positive bacterium Staphylococcus aureus.LsDef2 showed an antibacterial effect against S.aureus.LsCole and LsDef2 exhibited antibiofilm activity against S.aureus.The 2 AMPs disrupted cell membranes and caused leakage of S.aureus cell contents.The results indicated that the 3 AMPs in L.serricorne are involved in the innate immunity of this pest insect.These AMPs may have potential as antimicrobial agents for bacterial infection chemotherapy.Hence,data are discussed in relation to new control strategies with greater biosafety against pest insects with use of microbial biocontrol agents in combination with RNA interference against the insect's defensive AMP genes.

antimicrobial activityantimicrobial peptidesinnate immune responseLa-sioderma serricorneRNA interference

Yue Zhang、Yi Yan、Guy Smagghe、Hong Yang、Ren-huai Dai、Wen-jia Yang

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Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region,Institute of Entomology,Guizhou University,Guiyang,China

Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region,College of Biology and Environmental Engineering,Guiyang University,Guiyang,China

Program of Excellent Innovation Talents,Guizhou Province,ChinaGuizhou Province Graduate Research FundSpecial Project for Science and Technology Development of Local(Guizhou)under the Guidance of the Central Government

20206003-2YJSKYJJ[2021]053QKZYD[2022]4013

2024

10.1111/1744-7917.13298

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(4)
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