首页|Highly efficient expression of human extracellular superoxide dismutase(rhEcSOD)with ultraviolet-B-induced damage-resistance activity in transgenic silkworm cocoons

Highly efficient expression of human extracellular superoxide dismutase(rhEcSOD)with ultraviolet-B-induced damage-resistance activity in transgenic silkworm cocoons

扫码查看
原文链接
  • 万方数据
  • 维普
Extracellular superoxide dismutase(EcSOD)protects tissues from oxidative stress,and thus is considered as a therapeutic agent for many diseases such as atheroscle-rosis,hypertension,and cancer.However,cost-effective production of bioactive recombi-nant human EcSOD(rhEcSOD)remains a challenge.Herein,we developed an efficient strategy for producing active rhEcSOD by transgenic silkworms.rhEcSOD was success-fully synthesized as homodimers and homotetramers in the middle silk gland and spun into the cocoons with a concentration of 9.48±0.21 mg/g.Purification of rhEcSOD from the cocoons could be conveniently achieved with a purity of 99.50％and a yield of 3.5±0.5 mg/g.Additionally,N-glycosylation at the only site of N89 in rhEcSOD with 10 types were identified.The purified rhEcSOD gained the potent enzymatic activity of 4 162±293 U/mg after Cu/Zn ions incorporation.More importantly,rhEcSOD was ca-pable of penetrating and accumulating in the nuclei of cells to maintain cell morphology and attenuate ultraviolet B-induced cell apoptosis by eliminating reactive oxygen species and inhibiting the C-Jun N-terminal kinase signaling pathway.These results demonstrated that the transgenic silkworm could successfully produce rhEcSOD with enzymatic and biological activities for biomedical applications.

antioxidanthuman extracellular superoxide dismutaserecombinant expres-sionsilkworm silk gland

Feng Wang、Ri-Yuan Wang、De-Bin Zhong、Ping Zhao、Qing-You Xia

展开 >

Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City,Biological Science Research Center,Southwest University,Chongqing,China

Century Legend Biotechnology Research Institute(Chongqing)Co.,Ltd.,Chongqing,China

National Key Research and Development Program of ChinaChongqing Science and Technology CommissionChongqing Science and Technology CommissionYongchuan District Science and Technology Commission

2022YFD1201600cstc2020jcyjcxttX0001CSTB2022TIAD-KPX00832022ycyfjg20002

2024

10.1111/1744-7917.13289

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(4)