首页|A novel diagnostic gene region for distinguishing between two pest fruit flies:Bactrocera tryoni(Froggatt)and Bactrocera neohumeralis(Hardy)(Diptera:Tephritidae)

A novel diagnostic gene region for distinguishing between two pest fruit flies:Bactrocera tryoni(Froggatt)and Bactrocera neohumeralis(Hardy)(Diptera:Tephritidae)

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Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distri-butions,and host ranges.The need to differentiate between the two species is critical for accurate pest status assessment,management,biosecurity,and maintenance of reference colonies.While morphologically similar,adults may be separated based on subtle charac-ters;however,some characters exhibit intraspecific variability,creating overlap between the two species.Additionally,there is currently no single molecular marker or rapid diag-nostic assay that can reliably distinguish between B.neohumeralis and B.tryoni;therefore,ambiguous samples remain undiagnosed.Here we report the first molecular marker that can consistently distinguish between B.tryoni and B.neohumeralis.Our diagnostic region consists of two adjacent single nucleotide polymorphisms(SNPs)within the pangolin(pan)gene region.We confirmed the genotypes of each species are consistent across their distributional range,then developed a tetra-primer amplification refractory mutation sys-tem(ARMS)PCR assay for rapid diagnosis of the species.The assay utilizes four primers in multiplex,with two outer universal primers,and two internal primers:one designed to target two adjacent SNPs(AA)present in B.tryoni and the other targeting adjacent SNPs present in B.neohumeralis(GG).The assay accurately discriminates between the two species,but their SNP genotypes are shared with other nontarget tephritid fruit fly species.Therefore,this assay is most suited to adult diagnostics where species confirma-tion is necessary in determining ambiguous surveillance trap catches;maintaining pure colony lines;and in Sterile Insect Technique management responses.

ARMS-PCRcolonymolecular diagnosticspangolintetra-primer

Melissa L.Starkie、Elizabeth V.Fowler、Alexander M.Piper、Xiaocheng Zhu、Pauline Wyatt、David Gopurenko、Matt N.Krosch、Francesca Strutt、Karen F.Armstrong、Hamish Patrick、Mark K.Schutze、Mark J.Blacket

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Department of Agriculture and Fisheries,Biosecurity,Brisbane,Queensland,Australia

Agriculture Victoria,AgriBio,Bundoora,Victoria,Australia

Department of Primary Industries,Wagga Wagga Agricultural Institute,Wagga Wagga,New South Wales,Australia

Forensic Services Group,Queensland Police Service,Brisbane,Queensland,Australia

Department of Pest-management and Conservation,Lincoln University,Lincoln,New Zealand

Better Border Biosecurity(B3,B3nz.org.nz),Lincoln,New Zealand

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Tertiary Education Commission of New ZealandStrengthening Australia's Fruit Fly System Research ProgramAustralian Government,with contributions matched from state and territory governments

2024

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子:0.484
ISSN:1672-9609
年,卷(期):2024.31(4)