首页|Comparative analysis of locomotor behavior and head diurnal transcriptome regulation by PERIOD and CRY2 in the diamondback moth

Comparative analysis of locomotor behavior and head diurnal transcriptome regulation by PERIOD and CRY2 in the diamondback moth

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Earth's rotation shapes a 24-h cycle,governing circadian rhythms in organ-isms.In mammals,the core clock genes,CLOCK and BMAL1,are regulated by PERIODs(PERs)and CRYPTOCHROMEs(CRYs),but their roles remain unclear in the diamond-back moth,Plutella xylostella.To explore this,we studied P xylostella,which possesses a simplified circadian system compared to mammals.In P xylostella,we observed rhyth-mic expressions of the Pxper and Pxcry2 genes in their heads,with differing phases.In vitro experiments revealed that PxCRY2 repressed monarch butterfly CLK:BMAL1 tran-scriptional activation,while PxPER and other CRY-like proteins did not.However,PxPER showed an inhibitory effect on PxCLK/PxCYCLE.Using CRISPR/Cas9,we individually and in combination knocked out Pxper and Pxcry2,then conducted gene function studies and circadian transcriptome sequencing.Loss of either Pxper or Pxcry2 eliminated the activity peak after lights-off in light-dark cycles,and Pxcry2 loss reduced overall activ-ity.Pxcry2 was crucial for maintaining endogenous rhythms in constant darkness.Under light-dark conditions,1 098 genes exhibited rhythmic expression in wild-type P xylostella heads,with 749 relying on Pxper and Pxcry2 for their rhythms.Most core clock genes lost their rhythmicity in Pxper and Pxcry2 mutants,while Pxcry2 sustained rhythmic expres-sion,albeit with reduced amplitude and altered phase.Additionally,rhythmic genes were linked to biological processes like the spliceosome and Toll signaling pathway,with these rhythms depending on Pxper or Pxcry2 function.In summary,our study unveils differ-ences in circadian rhythm regulation by Pxper and Pxcry2 in P xylostella.This provides a valuable model for understanding circadian clock regulation in nocturnal animals.

CRY2daily transcriptomelocomotor behaviorPERIODPlutella xy-lostella

Wenfeng Chen、Danfeng Wang、Lingqi Yu、Wenmiao Zhong、Yao Yuan、Guang Yang

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Institute of Life Sciences,College of Biological Science and Engineering,Fuzhou University,Fuzhou,China

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Institute of Applied Ecology,Fujian Agriculture and Forestry University,Fuzhou,China

Joint International Research Laboratory of Ecological Pest Control,Ministry of Education,Fuzhou,China

Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops,Ministry of Agriculture,Fuzhou,China

Key Laboratory of Green Pest Control(Fujian Agriculture and Forestry University),Fujian Province University,Fuzhou,China

Ministerial and Provin

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2024

10.1111/1744-7917.13344

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(6)